biostudies-literatureKrishnan NRajiv Gandhi Centre for Biotechnology, Department of Biotechnology, Ministry of Science and Technology, IndiaUniversity Grants CommissionIndian Council of Medical Researche37064https://www.ebi.ac.uk/biostudies/studies/S-EPMC11403530biostudies-literatureUnknown10(17)<h4>Objective</h4>The role of β-hCG in breast cancer is largely unknown, this study aims to analyse the gene expression and clinical implications of β-hCG and its isoforms in various cancers focussing particularly in Breast Invasive Carcinoma (BRCA). A mechanistic approach deciphering the transcriptional regulation of β-hCG by BRCA1 was also explored.<h4>Methods</h4>Data from various comprehensive gene expression platforms like UALCAN, GEPIA2, GENT2, TIMER2, LinkedOmics, and STRING were used to analyse the expression of β-hCG and its clinical implications; Immunohistochemistry and ELISA for β-hCG expression analysis from human breast cancer patients; Electrophoretic mobility shift assay (EMSA) to analyse the direct binding of BRCA1 on β-hCG; Immunoblotting and Luciferase assay to understanHeliyonDirect DNA binding by BRCA1 on β-hCG promoter and its clinical implications.PMC11403530EMDR/SGll3l 2023-2686Anandan JRajan AV G MS PMohammed SWarrier AVSundaram SKrishnan NNair RSIttycheria SSYadev IKumari PSrinivas PR L NfalseDirect DNA binding by BRCA1 on β-hCG promoter and its clinical implications.<h4>Objective</h4>The role of β-hCG in breast cancer is largely unknown, this study aims to analyse the gene expression and clinical implications of β-hCG and its isoforms in various cancers focussing particularly in Breast Invasive Carcinoma (BRCA). A mechanistic approach deciphering the transcriptional regulation of β-hCG by BRCA1 was also explored.<h4>Methods</h4>Data from various comprehensive gene expression platforms like UALCAN, GEPIA2, GENT2, TIMER2, LinkedOmics, and STRING were used to analyse the expression of β-hCG and its clinical implications; Immunohistochemistry and ELISA for β-hCG expression analysis from human breast cancer patients; Electrophoretic mobility shift assay (EMSA) to analyse the direct binding of BRCA1 on β-hCG; Immunoblotting and Luciferase assay to understan2024-01-01T00:00:00Z2024 Sep2026-06-03T16:56:39.438Z2025-04-04T02:54:09.024ZS-EPMC114035303928606510.1016/j.heliyon.2024.e37064