biostudies-literatureUnknown72(36)Pieczonka SADistelh?user Brauerei Ernst Bauer GmbH & Co KGThe removal of husks before the mashing process, also known as the Kubessa method, is an established brewing practice often positively associated with smoothness and better flavor-stability of beer. Empirical evidence on the effect of the Kubessa method on beer, however, has been lacking. Similarly, our study's comprehensive analysis of established brewing attributes revealed that traditional methods do not fully capture the impact of husk separation in beer brewing. Conclusive evidence of the Kubessa method's impact on beer aging chemistry was obtained through ultrahigh resolution mass spectrometry (FT-ICR-MS), revealing intricate molecular details inaccessible to conventional analytical techniques. The compositional information on thousands of molecules in Kubessa beer was resolved and cJournal of agricultural and food chemistry20048-20055https://www.ebi.ac.uk/biostudies/studies/S-EPMC11403607biostudies-literatureHusk Separation (Kubessa Method) Impacts the Aging Chemistry of Beer.PMC11403607Lehnhardt FBrauer JRychlik MEiken JGastl MWachtler AWeidner LZarnkow MSchmitt-Kopplin PPieczonka SABrass LfalseHusk Separation (Kubessa Method) Impacts the Aging Chemistry of Beer.The removal of husks before the mashing process, also known as the Kubessa method, is an established brewing practice often positively associated with smoothness and better flavor-stability of beer. Empirical evidence on the effect of the Kubessa method on beer, however, has been lacking. Similarly, our study's comprehensive analysis of established brewing attributes revealed that traditional methods do not fully capture the impact of husk separation in beer brewing. Conclusive evidence of the Kubessa method's impact on beer aging chemistry was obtained through ultrahigh resolution mass spectrometry (FT-ICR-MS), revealing intricate molecular details inaccessible to conventional analytical techniques. The compositional information on thousands of molecules in Kubessa beer was resolved and c2024-01-01T00:00:00Z2024 Sep2025-04-21T22:19:26.25Z2025-04-05T18:43:19.25ZS-EPMC114036073921910210.1021/acs.jafc.4c05099