biostudies-literatureBell TAHelen Hay Whitney FoundationNational Institute of General Medical SciencesNIGMS NIH HHSe100061https://www.ebi.ac.uk/biostudies/studies/S-EPMC11405016biostudies-literatureUnknown13Prominin 1 (Prom1) is a five-transmembrane pass integral membrane protein that associates with curved regions of the plasma membrane. Prom1 interacts with membrane cholesterol and actively remodels the plasma membrane. Membrane-bending activity is particularly evident in photoreceptors, where Prom1 loss-of-function mutations cause failure of outer segment homeostasis, leading to cone-rod retinal dystrophy (CRRD). The Tweety Homology (Ttyh) protein family has been proposed to be homologous to Prominin, but it is not known whether Ttyh proteins have an analogous membrane-bending function. Here, we characterize the membrane-bending activity of human Prom1 and Ttyh1 in native bilayer membranes. We find that Prom1 and Ttyh1 both induce formation of extracellular vesicles (EVs) in cultured mammaeLifeProminin 1 and Tweety Homology 1 both induce extracellular vesicle formation.PMC11405016R35 GM142553R35GM142553Monshizadeh ABell TANguyen THDardari HLuce BEChao LHAnanda VYHakim PfalseProminin 1 and Tweety Homology 1 both induce extracellular vesicle formation.Prominin 1 (Prom1) is a five-transmembrane pass integral membrane protein that associates with curved regions of the plasma membrane. Prom1 interacts with membrane cholesterol and actively remodels the plasma membrane. Membrane-bending activity is particularly evident in photoreceptors, where Prom1 loss-of-function mutations cause failure of outer segment homeostasis, leading to cone-rod retinal dystrophy (CRRD). The Tweety Homology (Ttyh) protein family has been proposed to be homologous to Prominin, but it is not known whether Ttyh proteins have an analogous membrane-bending function. Here, we characterize the membrane-bending activity of human Prom1 and Ttyh1 in native bilayer membranes. We find that Prom1 and Ttyh1 both induce formation of extracellular vesicles (EVs) in cultured mamma2024-01-01T00:00:00Z2024 Aug2026-06-02T23:19:17.631Z2025-04-04T01:18:13.35ZS-EPMC114050163913655410.7554/eLife.100061