biostudies-literatureTao ZState Key Laboratory of Hybrid Rice8107https://www.ebi.ac.uk/biostudies/studies/S-EPMC11405696biostudies-literatureUnknown15(1)Brown planthopper (BPH) is the most destructive insect pest of rice. Drought is the most detrimental environmental stress. BPH infestation causes adaxial leaf-rolling and bulliform cells (BCs) shrinkage similar to drought. The BC-related abaxially curled leaf1 (ACL1) gene negatively regulates BPH resistance and drought tolerance, with decreased cuticular wax in the gain-of-function mutant ACL1-D. ACL1 shows an epidermis-specific expression. The TurboID system and multiple biochemical assays reveal that ACL1 interacts with the epidermal-characteristic rice outermost cell-specific (ROC) proteins. ROC4 and ROC5 positively regulate BPH resistance and drought tolerance through modulating cuticular wax and BCs, respectively. Overexpression of ROC4 and ROC5 both rescue ACL1-D mutant in various reNature communicationsACL1-ROC4/5 complex reveals a common mechanism in rice response to brown planthopper infestation and drought.PMC11405696KF201805Liu XTao ZLi HLi DZhu LSun BHu WWang SMiao XShi ZfalseACL1-ROC4/5 complex reveals a common mechanism in rice response to brown planthopper infestation and drought.Brown planthopper (BPH) is the most destructive insect pest of rice. Drought is the most detrimental environmental stress. BPH infestation causes adaxial leaf-rolling and bulliform cells (BCs) shrinkage similar to drought. The BC-related abaxially curled leaf1 (ACL1) gene negatively regulates BPH resistance and drought tolerance, with decreased cuticular wax in the gain-of-function mutant ACL1-D. ACL1 shows an epidermis-specific expression. The TurboID system and multiple biochemical assays reveal that ACL1 interacts with the epidermal-characteristic rice outermost cell-specific (ROC) proteins. ROC4 and ROC5 positively regulate BPH resistance and drought tolerance through modulating cuticular wax and BCs, respectively. Overexpression of ROC4 and ROC5 both rescue ACL1-D mutant in various re2024-01-01T00:00:00Z2024 Sep2026-06-03T21:18:14.436Z2025-04-05T18:45:35.843ZS-EPMC114056963928517110.1038/s41467-024-52436-w