{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lin J"],"funding":["NCI NIH HHS","NIGMS NIH HHS"],"pagination":["5314-5327"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11407235"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(9)"],"pubmed_abstract":["We present a mechanically sheared image acquisition format for upright and open-top light-sheet microscopes that automatically places data in its proper spatial context. This approach, which reduces computational post-processing and eliminates unnecessary interpolation or duplication of the data, is demonstrated on an upright variant of axially swept light-sheet microscopy (ASLM) that achieves a field of view, measuring 774 × 435 microns, that is 3.2-fold larger than previous models and a raw and isotropic resolution of ∼460 nm. Combined, we demonstrate the power of this approach by imaging sub-diffraction beads, cleared biological tissues, and expanded specimens."],"journal":["Biomedical optics express"],"pubmed_title":["Mechanically sheared axially swept light-sheet microscopy."],"pmcid":["PMC11407235"],"funding_grant_id":["RM1 GM145399","U54 CA268072"],"pubmed_authors":["Marin Z","Borges HM","Abbott DH","Shen Q","Lin J","Mehra D","Wang X","Galecki S","Dean KM","Haug J"],"additional_accession":[]},"is_claimable":false,"name":"Mechanically sheared axially swept light-sheet microscopy.","description":"We present a mechanically sheared image acquisition format for upright and open-top light-sheet microscopes that automatically places data in its proper spatial context. This approach, which reduces computational post-processing and eliminates unnecessary interpolation or duplication of the data, is demonstrated on an upright variant of axially swept light-sheet microscopy (ASLM) that achieves a field of view, measuring 774 × 435 microns, that is 3.2-fold larger than previous models and a raw and isotropic resolution of ∼460 nm. Combined, we demonstrate the power of this approach by imaging sub-diffraction beads, cleared biological tissues, and expanded specimens.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2026-06-03T22:37:37.223Z","creation":"2025-04-06T00:56:39.381Z"},"accession":"S-EPMC11407235","cross_references":{"pubmed":["39296406"],"doi":["10.1364/BOE.526145"]}}