biostudies-literatureChatzimichail SNational Institute for Health Research Health Protection Research UnitOxford Martin School, University of OxfordJohn Fell Fund, University of OxfordNIHR Oxford Biomedical Research CentreNational Institute for Health Research (NIHR)Wellcome TrustBiotechnology and Biological Sciences Research CouncilEngineering and Physical Sciences Research Council4843-4858https://www.ebi.ac.uk/biostudies/studies/S-EPMC11409657biostudies-literatureUnknown24(20)We demonstrate the rapid capture, enrichment, and identification of bacterial pathogens using Adaptive Channel Bacterial Capture (ACBC) devices. Using controlled tuning of device backpressure in polydimethylsiloxane (PDMS) devices, we enable the controlled formation of capture regions capable of trapping bacteria from low cell density samples with near 100% capture efficiency. The technical demands to prepare such devices are much lower compared to conventional methods for bacterial trapping and can be achieved with simple benchtop fabrication methods. We demonstrate the capture and identification of seven species of bacteria with bacterial concentrations lower than 1000 cells per mL, including common Gram-negative and Gram-positive pathogens such as <i>Escherichia coli</i> and <i>StaphyloLab on a chipRapid identification of bacterial isolates using microfluidic adaptive channels and multiplexed fluorescence microscopy.PMC11409657Imaging for Rapid Antimicrobial Resistance TestingBB/N018656/1BB/S008896/1NIHR200915EP/X525777/100123790008776110164/Z/15/ZChatzimichail SFeehily CFarrar AKyropoulos JNellaker COakley SAndersson MEl Sayyed HKapanidis ANBarrett LStoesser NCrook DTurner PfalseRapid identification of bacterial isolates using microfluidic adaptive channels and multiplexed fluorescence microscopy.We demonstrate the rapid capture, enrichment, and identification of bacterial pathogens using Adaptive Channel Bacterial Capture (ACBC) devices. Using controlled tuning of device backpressure in polydimethylsiloxane (PDMS) devices, we enable the controlled formation of capture regions capable of trapping bacteria from low cell density samples with near 100% capture efficiency. The technical demands to prepare such devices are much lower compared to conventional methods for bacterial trapping and can be achieved with simple benchtop fabrication methods. We demonstrate the capture and identification of seven species of bacteria with bacterial concentrations lower than 1000 cells per mL, including common Gram-negative and Gram-positive pathogens such as <i>Escherichia coli</i> and <i>Staphylo2024-01-01T00:00:00Z2024 Oct2026-07-14T20:37:44.259Z2025-04-04T01:54:46.505ZS-EPMC114096573929184710.1039/d4lc00325j