biostudies-literatureUnknown9(37)Jing WThe <i>Mycoplasma pneumoniae</i> outbreak poses health risks to community residents. However, it still has limitations for current clinical diagnostic methods (qPCR nucleic acid assay or IgM immunoassay), including specialized handling, expensive equipment, prolonged turnaround time, and false positives and negatives, highlighting the need to improve clinical diagnostic methods. Herein, we present a novel centrifugal microfluidics-based method for rapidly diagnosing <i>M. pneumoniae</i> infections (CHAMP system). This user-friendly method combines CRISPR/Cas12b and real-time loop-mediated isothermal amplification (LAMP) in a one-pot reaction, offering high sensitivity, specificity, and simplicity for methodology. By adding fully automated nucleic acid magnetic bead-extracted samples to a pACS omega38989-38997https://www.ebi.ac.uk/biostudies/studies/S-EPMC11411642biostudies-literatureCHAMP: A Centrifugal Microfluidics-Based CRISPR/Cas12b-Combined Real-Time LAMP One-Pot Method for <i>Mycoplasma pneumoniae</i> Infection Diagnosis.PMC11411642Li XZhang TFeng MMin XSui GLuo LCheng XJing WJin KfalseCHAMP: A Centrifugal Microfluidics-Based CRISPR/Cas12b-Combined Real-Time LAMP One-Pot Method for <i>Mycoplasma pneumoniae</i> Infection Diagnosis.The <i>Mycoplasma pneumoniae</i> outbreak poses health risks to community residents. However, it still has limitations for current clinical diagnostic methods (qPCR nucleic acid assay or IgM immunoassay), including specialized handling, expensive equipment, prolonged turnaround time, and false positives and negatives, highlighting the need to improve clinical diagnostic methods. Herein, we present a novel centrifugal microfluidics-based method for rapidly diagnosing <i>M. pneumoniae</i> infections (CHAMP system). This user-friendly method combines CRISPR/Cas12b and real-time loop-mediated isothermal amplification (LAMP) in a one-pot reaction, offering high sensitivity, specificity, and simplicity for methodology. By adding fully automated nucleic acid magnetic bead-extracted samples to a p2024-01-01T00:00:00Z2024 Sep2026-06-04T09:42:21.763Z2025-04-06T16:24:43.953ZS-EPMC114116423931012910.1021/acsomega.4c05489