biostudies-literatureLi TFundamental Research Funds for the Central UniversitiesPostdoctoral Fellowship Program of CPSF under GrantNational Natural Science Foundation of ChinaNatural Science Foundation of Jiangsu Province122https://www.ebi.ac.uk/biostudies/studies/S-EPMC11411985biostudies-literatureUnknown17(1)<h4>Background</h4>The ability of lignocellulose degradation for filamentous fungi is always attributed to their efficient CAZymes system with broader applications in bioenergy development. ADP-ribosylation factor GTPase-activating proteins (Arf-GAPs), pivotal in fungal morphogenesis, lack comprehensive studies on their regulatory mechanisms in lignocellulose utilization.<h4>Results</h4>Here, the orthologs (TgGlo3 and TgGcs1) of Arf-GAPs in S. cerevisiae were characterized in Trichoderma guizhouense NJAU4742. The results indicated that overexpression of Tggcs1 (OE-Tggcs1) enhanced the lignocellulose utilization, whereas increased expression of Tgglo3 (OE-Tgglo3) elicited antithetical responses. On the fourth day of fermentation with rice straw as the sole carbon source, the activities of eBiotechnology for biofuels and bioproductsDivergent roles of ADP-ribosylation factor GTPase-activating proteins in lignocellulose utilization of Trichoderma guizhouense NJAU4742.PMC11411985BK2023099132302679GZC2023112632172680XUEKEN2023039Cao LLiu YWang QShen QLi TZhu HLiu DWang JfalseDivergent roles of ADP-ribosylation factor GTPase-activating proteins in lignocellulose utilization of Trichoderma guizhouense NJAU4742.<h4>Background</h4>The ability of lignocellulose degradation for filamentous fungi is always attributed to their efficient CAZymes system with broader applications in bioenergy development. ADP-ribosylation factor GTPase-activating proteins (Arf-GAPs), pivotal in fungal morphogenesis, lack comprehensive studies on their regulatory mechanisms in lignocellulose utilization.<h4>Results</h4>Here, the orthologs (TgGlo3 and TgGcs1) of Arf-GAPs in S. cerevisiae were characterized in Trichoderma guizhouense NJAU4742. The results indicated that overexpression of Tggcs1 (OE-Tggcs1) enhanced the lignocellulose utilization, whereas increased expression of Tgglo3 (OE-Tgglo3) elicited antithetical responses. On the fourth day of fermentation with rice straw as the sole carbon source, the activities of e2024-01-01T00:00:00Z2024 Sep2026-06-01T13:44:37.067Z2025-04-04T12:54:15.18ZS-EPMC114119853929471210.1186/s13068-024-02570-w