{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pitino MA"],"funding":["NICHD NIH HHS","Family Larsson-Rosenquist Foundation","National Institutes of Health","Canadian Institutes of Health Research","NIH HHS"],"pagination":["140973"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11413619"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["462"],"pubmed_abstract":["High-pressure processing (HPP) of donor human milk (DM) minimally impacts the concentration and bioactivity of some important bioactive proteins including lactoferrin, and bile salt-stimulated lipase (BSSL) compared to Holder pasteurization (HoP), yet the impact of HPP and subsequent digestion on the full array of proteins detectable by proteomics remains unclear. We investigated how HPP impacts undigested proteins in DM post-processing and across digestion by proteomic analysis. Each pool of milk (n = 3) remained raw, or was treated by HPP (500 MPa, 10 min) or HoP (62.5 °C, 30 min), and underwent dynamic in vitro digestion simulating the preterm infant. In the meal, major proteins were minimally changed post-processing. HPP-treated milk proteins better resisted proximal digestion (except "],"journal":["Food chemistry"],"pubmed_title":["Comparative proteomic analysis of donor human milk treated by high-pressure processing or Holder pasteurization on undigested proteins across dynamic simulated preterm infant digestion."],"pmcid":["PMC11413619"],"funding_grant_id":["S10 OD020111","R01 HD106140","R01 HD097367"],"pubmed_authors":["O'Connor DL","Doyen A","Pouliot Y","Dallas DC","Wazed MA","Pitino MA","Kim BJ","Stone D","Unger S","Kumar S"],"additional_accession":[]},"is_claimable":false,"name":"Comparative proteomic analysis of donor human milk treated by high-pressure processing or Holder pasteurization on undigested proteins across dynamic simulated preterm infant digestion.","description":"High-pressure processing (HPP) of donor human milk (DM) minimally impacts the concentration and bioactivity of some important bioactive proteins including lactoferrin, and bile salt-stimulated lipase (BSSL) compared to Holder pasteurization (HoP), yet the impact of HPP and subsequent digestion on the full array of proteins detectable by proteomics remains unclear. We investigated how HPP impacts undigested proteins in DM post-processing and across digestion by proteomic analysis. Each pool of milk (n = 3) remained raw, or was treated by HPP (500 MPa, 10 min) or HoP (62.5 °C, 30 min), and underwent dynamic in vitro digestion simulating the preterm infant. In the meal, major proteins were minimally changed post-processing. HPP-treated milk proteins better resisted proximal digestion (except ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2026-06-09T05:04:12.257Z","creation":"2026-06-09T03:07:26.695Z"},"accession":"S-EPMC11413619","cross_references":{"pubmed":["39208730"],"doi":["10.1016/j.foodchem.2024.140973"]}}