biostudies-literatureBojmar LNCATS NIH HHSNIDDK NIH HHSCancerfonden (Swedish Cancer Society)NIAID NIH HHSVetenskapsrådet (Swedish Research Council)NCI NIH HHSSvenska Sällskapet för Medicinsk Forskning (Swedish Society for Medical Research)2170-2180https://www.ebi.ac.uk/biostudies/studies/S-EPMC11416063biostudies-literatureUnknown30(8)Metastasis occurs frequently after resection of pancreatic cancer (PaC). In this study, we hypothesized that multi-parametric analysis of pre-metastatic liver biopsies would classify patients according to their metastatic risk, timing and organ site. Liver biopsies obtained during pancreatectomy from 49 patients with localized PaC and 19 control patients with non-cancerous pancreatic lesions were analyzed, combining metabolomic, tissue and single-cell transcriptomics and multiplex imaging approaches. Patients were followed prospectively (median 3 years) and classified into four recurrence groups; early (<6 months after resection) or late (>6 months after resection) liver metastasis (LiM); extrahepatic metastasis (EHM); and disease-free survivors (no evidence of disease (NED)). Overall, PaCNature medicineMulti-parametric atlas of the pre-metastatic liver for prediction of metastatic outcome in early-stage pancreatic cancer.PMC11416063U01 CA169538UL1 TR002384TG-23-0306P30 CA008748UL1 TR000457R01 CA169416U01 CA224175U54 CA163117R01 CA21851321 1824 Pj 01 HR01 DK121072R01 CA234614U54 CA1631202021-600 02356R35 CA232093U01 CA210240R50 CA211462grant no. S21-0079U19 AI144301R01 CA207983O'Reilly EMKingham TPZhang HHoshino ARajasekhar VKFirmenich LScherz AHanna SKelsen DJonsson ASigel CJonsson CDeMatteo RPCioffi MHollingsworth MALauritzen PChoubey AHernandez DBromberg JElemento OBuehring WVelasco Riestra PBlomstrand HJessurun JVincent CTAllen PJSandstrom PSimeone DMGlidden CAskan GNasar NBalachandran VPChakraborty JJarnagin WRFriedman MKeshari KBram YSrouji RYarden YScherz-Shouval RHealey JHEgeblad MHernandez JMJohnson KEKim JMutvei APMatei IRSagi ITuveson DAZheng JSimpson ALCosta-Silva BPaul DMRavichandran HOren MSong YBjornsson BLyden DArarso YGrandgenett PMvan Beek EVinagolu-Baur JPBasturk OBoudreau NKim HSJolissaint JSZambirinis CPSchwartz REBurman JLieberman GD'Angelica MIShaashua LLewis JSIacobuzio-Donahue CAMejia DLBojmar LfalseMulti-parametric atlas of the pre-metastatic liver for prediction of metastatic outcome in early-stage pancreatic cancer.Metastasis occurs frequently after resection of pancreatic cancer (PaC). In this study, we hypothesized that multi-parametric analysis of pre-metastatic liver biopsies would classify patients according to their metastatic risk, timing and organ site. Liver biopsies obtained during pancreatectomy from 49 patients with localized PaC and 19 control patients with non-cancerous pancreatic lesions were analyzed, combining metabolomic, tissue and single-cell transcriptomics and multiplex imaging approaches. Patients were followed prospectively (median 3 years) and classified into four recurrence groups; early (<6 months after resection) or late (>6 months after resection) liver metastasis (LiM); extrahepatic metastasis (EHM); and disease-free survivors (no evidence of disease (NED)). Overall, PaC2024-01-01T00:00:00Z2024 Aug2026-06-02T16:10:59.508Z2025-04-06T22:30:21.868ZS-EPMC114160633894299210.1038/s41591-024-03075-7