{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bali P"],"funding":["US Department of Defense","NIGMS NIH HHS"],"pagination":["103302"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11416640"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5(3)"],"pubmed_abstract":["Helicobacter-induced gastric cancer progresses very slowly, even in animal models, making it difficult to study. Here, we present a protocol to establish an accelerated murine model for Helicobacter-induced gastric cancer. We describe steps for infecting mice with Helicobacter felis, harvesting gastric tissue, assessing disease severity by histopathologic scoring, and performing gene expression studies with RT-qPCR and RNA sequencing. The accelerated model shows rapid progression of the disease, with gastric precancerous lesions developing within 6 months post-infection with Helicobacter. For complete details on the use and execution of this protocol, please refer to Bali et al.<sup>1</sup>."],"journal":["STAR protocols"],"pubmed_title":["Protocol to establish an accelerated murine model for Helicobacter-induced gastric cancer."],"pmcid":["PMC11416640"],"funding_grant_id":["R01 GM134366","W81XWH-20-1-0675","R35 GM149520"],"pubmed_authors":["Hernandez J","Bali P","Obonyo M","Benner C","Lozano-Pope I","Estrada MV"],"additional_accession":[]},"is_claimable":false,"name":"Protocol to establish an accelerated murine model for Helicobacter-induced gastric cancer.","description":"Helicobacter-induced gastric cancer progresses very slowly, even in animal models, making it difficult to study. Here, we present a protocol to establish an accelerated murine model for Helicobacter-induced gastric cancer. We describe steps for infecting mice with Helicobacter felis, harvesting gastric tissue, assessing disease severity by histopathologic scoring, and performing gene expression studies with RT-qPCR and RNA sequencing. The accelerated model shows rapid progression of the disease, with gastric precancerous lesions developing within 6 months post-infection with Helicobacter. For complete details on the use and execution of this protocol, please refer to Bali et al.<sup>1</sup>.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2026-06-02T23:15:20.753Z","creation":"2025-04-19T19:32:37.243Z"},"accession":"S-EPMC11416640","cross_references":{"pubmed":["39264805"],"doi":["10.1016/j.xpro.2024.103302"]}}