biostudies-literatureBali PUS Department of DefenseNIGMS NIH HHS103302https://www.ebi.ac.uk/biostudies/studies/S-EPMC11416640biostudies-literatureUnknown5(3)Helicobacter-induced gastric cancer progresses very slowly, even in animal models, making it difficult to study. Here, we present a protocol to establish an accelerated murine model for Helicobacter-induced gastric cancer. We describe steps for infecting mice with Helicobacter felis, harvesting gastric tissue, assessing disease severity by histopathologic scoring, and performing gene expression studies with RT-qPCR and RNA sequencing. The accelerated model shows rapid progression of the disease, with gastric precancerous lesions developing within 6 months post-infection with Helicobacter. For complete details on the use and execution of this protocol, please refer to Bali et al.¹.STAR protocolsProtocol to establish an accelerated murine model for Helicobacter-induced gastric cancer.PMC11416640R01 GM134366W81XWH-20-1-0675R35 GM149520Hernandez JBali PObonyo MBenner CLozano-Pope IEstrada MVfalseProtocol to establish an accelerated murine model for Helicobacter-induced gastric cancer.Helicobacter-induced gastric cancer progresses very slowly, even in animal models, making it difficult to study. Here, we present a protocol to establish an accelerated murine model for Helicobacter-induced gastric cancer. We describe steps for infecting mice with Helicobacter felis, harvesting gastric tissue, assessing disease severity by histopathologic scoring, and performing gene expression studies with RT-qPCR and RNA sequencing. The accelerated model shows rapid progression of the disease, with gastric precancerous lesions developing within 6 months post-infection with Helicobacter. For complete details on the use and execution of this protocol, please refer to Bali et al.¹.2024-01-01T00:00:00Z2024 Sep2026-06-02T23:15:20.753Z2025-04-19T19:32:37.243ZS-EPMC114166403926480510.1016/j.xpro.2024.103302