biostudies-literatureUnknownKim SKNIGMS NIH HHSNIH HHSMicrotubule (MT) regulation is essential for oocyte development. In <i>Drosophila</i>, MT stability, polarity, abundance, and orientation undergo dynamic changes across developmental stages. In our effort to identify novel microtubule-associated proteins (MAPs) that regulate MTs in the <i>Drosophila</i> ovary, we identified a previously uncharacterized gene, CG18190, encoding a novel MT end-binding (EB) protein, which we propose to name EB-SUN. We show that EB-SUN colocalizes with EB1 at growing microtubule plus-ends in <i>Drosophila</i> S2 cells. Tissue-specific and developmental expression profiles from Paralog Explorer reveal that EB-SUN is predominantly expressed in the ovary and early embryos, while EB1 is ubiquitously expressed. Furthermore, as early as oocyte determination, EB-SUN cbioRxiv : the preprint server for biology2024.09.11.612465https://www.ebi.ac.uk/biostudies/studies/S-EPMC11419005biostudies-literatureEB-SUN, a New Microtubule Plus-End Tracking Protein in &lt;i&gt;Drosophila&lt;/i&gt;.PMC11419005R35 GM131752P40 OD018537Lu WLee BSRogers SLKim SKGelfand VIfalseEB-SUN, a New Microtubule Plus-End Tracking Protein in &lt;i&gt;Drosophila&lt;/i&gt;.Microtubule (MT) regulation is essential for oocyte development. In <i>Drosophila</i>, MT stability, polarity, abundance, and orientation undergo dynamic changes across developmental stages. In our effort to identify novel microtubule-associated proteins (MAPs) that regulate MTs in the <i>Drosophila</i> ovary, we identified a previously uncharacterized gene, CG18190, encoding a novel MT end-binding (EB) protein, which we propose to name EB-SUN. We show that EB-SUN colocalizes with EB1 at growing microtubule plus-ends in <i>Drosophila</i> S2 cells. Tissue-specific and developmental expression profiles from Paralog Explorer reveal that EB-SUN is predominantly expressed in the ovary and early embryos, while EB1 is ubiquitously expressed. Furthermore, as early as oocyte determination, EB-SUN c2024-01-01T00:00:00Z2024 Sep2025-04-19T18:15:00.031Z2025-04-19T18:15:00.031ZS-EPMC114190053931433810.1101/2024.09.11.612465