{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Guinard I"],"funding":["Agence Nationale de la Recherche (ANR)","Agence Nationale de la Recherche"],"pagination":["e2407829121"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11420179"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["121(38)"],"pubmed_abstract":["Blood platelets are produced by megakaryocytes (MKs), their parent cells, which are in the bone marrow. Once mature, MK pierces through the sinusoid vessel, and the initial protrusion further elongates as proplatelet or buds to release platelets. The mechanisms controlling the decision to initiate proplatelet and platelet formation are unknown. Here, we show that the mechanical properties of the microenvironment prevent proplatelet and platelet release in the marrow stroma while allowing this process in the bloodstream. Loss of marrow confinement following myelosuppression led to inappropriate proplatelet and platelet release into the extravascular space. We further used an inert viscoelastic hydrogel to evaluate the impact of compressive stress. Transcriptional analysis showed that cultur"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Mechanical confinement prevents ectopic platelet release."],"pmcid":["PMC11420179"],"funding_grant_id":["ANR 18-CE14-0037"],"pubmed_authors":["Weber J","Brassard-Jollive N","Ruch L","Leon C","Guinard I","Boscher J","Eckly A"],"additional_accession":[]},"is_claimable":false,"name":"Mechanical confinement prevents ectopic platelet release.","description":"Blood platelets are produced by megakaryocytes (MKs), their parent cells, which are in the bone marrow. Once mature, MK pierces through the sinusoid vessel, and the initial protrusion further elongates as proplatelet or buds to release platelets. The mechanisms controlling the decision to initiate proplatelet and platelet formation are unknown. Here, we show that the mechanical properties of the microenvironment prevent proplatelet and platelet release in the marrow stroma while allowing this process in the bloodstream. Loss of marrow confinement following myelosuppression led to inappropriate proplatelet and platelet release into the extravascular space. We further used an inert viscoelastic hydrogel to evaluate the impact of compressive stress. Transcriptional analysis showed that cultur","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2026-05-02T14:22:46.665Z","creation":"2025-04-06T10:34:22.538Z"},"accession":"S-EPMC11420179","cross_references":{"pubmed":["39236232"],"doi":["10.1073/pnas.2407829121"]}}