{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pohlmann S"],"funding":["Swiss National Science Foundation","NIAID NIH HHS","PHS HHS"],"pagination":["4664-72"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC114220"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["75(10)"],"pubmed_abstract":["Dendritic cells (DCs) efficiently bind and transmit human immunodeficiency virus (HIV) to cocultured T cells and so may play an important role in HIV transmission. DC-SIGN, a novel C-type lectin that is expressed in DCs, has recently been shown to bind R5 HIV type 1 (HIV-1) strains and a laboratory-adapted X4 strain. To characterize the interaction of DC-SIGN with primate lentiviruses, we investigated the structural determinants of DC-SIGN required for virus binding and transmission to permissive cells. We constructed a panel of DC-SIGN mutants and established conditions which allowed comparable cell surface expression of all mutants. We found that R5, X4, and R5X4 HIV-1 isolates as well as simian immunodeficiency and HIV-2 strains bound to DC-SIGN and could be transmitted to CD4/corecepto"],"journal":["Journal of virology"],"pubmed_title":["DC-SIGN interactions with human immunodeficiency virus type 1 and 2 and simian immunodeficiency virus."],"pmcid":["PMC114220"],"funding_grant_id":["P30-AI45008","AI 35383","611772","P30 AI045008","40880"],"pubmed_authors":["Baribaud F","Lee B","Leslie GJ","Doms RW","Munch J","Sanchez MD","Pohlmann S","Hiebenthal-Millow K","Kirchhoff F"],"additional_accession":[]},"is_claimable":false,"name":"DC-SIGN interactions with human immunodeficiency virus type 1 and 2 and simian immunodeficiency virus.","description":"Dendritic cells (DCs) efficiently bind and transmit human immunodeficiency virus (HIV) to cocultured T cells and so may play an important role in HIV transmission. DC-SIGN, a novel C-type lectin that is expressed in DCs, has recently been shown to bind R5 HIV type 1 (HIV-1) strains and a laboratory-adapted X4 strain. To characterize the interaction of DC-SIGN with primate lentiviruses, we investigated the structural determinants of DC-SIGN required for virus binding and transmission to permissive cells. We constructed a panel of DC-SIGN mutants and established conditions which allowed comparable cell surface expression of all mutants. We found that R5, X4, and R5X4 HIV-1 isolates as well as simian immunodeficiency and HIV-2 strains bound to DC-SIGN and could be transmitted to CD4/corecepto","dates":{"release":"2001-01-01T00:00:00Z","publication":"2001 May","modification":"2025-04-04T09:52:35.223Z","creation":"2019-03-27T00:17:05Z"},"accession":"S-EPMC114220","cross_references":{"pubmed":["11312337"],"doi":["10.1128/jvi.75.10.4664-4672.2001","10.1128/JVI.75.10.4664-4672.2001"]}}