biostudies-literaturePohlmann SSwiss National Science FoundationNIAID NIH HHSPHS HHS4664-72https://www.ebi.ac.uk/biostudies/studies/S-EPMC114220biostudies-literatureUnknown75(10)Dendritic cells (DCs) efficiently bind and transmit human immunodeficiency virus (HIV) to cocultured T cells and so may play an important role in HIV transmission. DC-SIGN, a novel C-type lectin that is expressed in DCs, has recently been shown to bind R5 HIV type 1 (HIV-1) strains and a laboratory-adapted X4 strain. To characterize the interaction of DC-SIGN with primate lentiviruses, we investigated the structural determinants of DC-SIGN required for virus binding and transmission to permissive cells. We constructed a panel of DC-SIGN mutants and established conditions which allowed comparable cell surface expression of all mutants. We found that R5, X4, and R5X4 HIV-1 isolates as well as simian immunodeficiency and HIV-2 strains bound to DC-SIGN and could be transmitted to CD4/coreceptoJournal of virologyDC-SIGN interactions with human immunodeficiency virus type 1 and 2 and simian immunodeficiency virus.PMC114220P30-AI45008AI 35383611772P30 AI04500840880Baribaud FLee BLeslie GJDoms RWMunch JSanchez MDPohlmann SHiebenthal-Millow KKirchhoff FfalseDC-SIGN interactions with human immunodeficiency virus type 1 and 2 and simian immunodeficiency virus.Dendritic cells (DCs) efficiently bind and transmit human immunodeficiency virus (HIV) to cocultured T cells and so may play an important role in HIV transmission. DC-SIGN, a novel C-type lectin that is expressed in DCs, has recently been shown to bind R5 HIV type 1 (HIV-1) strains and a laboratory-adapted X4 strain. To characterize the interaction of DC-SIGN with primate lentiviruses, we investigated the structural determinants of DC-SIGN required for virus binding and transmission to permissive cells. We constructed a panel of DC-SIGN mutants and established conditions which allowed comparable cell surface expression of all mutants. We found that R5, X4, and R5X4 HIV-1 isolates as well as simian immunodeficiency and HIV-2 strains bound to DC-SIGN and could be transmitted to CD4/corecepto2001-01-01T00:00:00Z2001 May2025-04-04T09:52:35.223Z2019-03-27T00:17:05ZS-EPMC1142201131233710.1128/jvi.75.10.4664-4672.200110.1128/JVI.75.10.4664-4672.2001