{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Morgan KJ"],"funding":["NHLBI NIH HHS","NINDS NIH HHS","NIAMS NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["The exploration of cell-type and environmentally-responsive nuclear pore complex (NPC) plasticity requires new, accessible tools. Using pan-Expansion Microscopy (pan-ExM), NPCs were identified by machine learning-facilitated segmentation with resolved cytoplasmic rings (CR), inner rings (IR) and nuclear rings (NR). They exhibited a large range of diameters with a bias for dilated NPCs at the basal nuclear surface in clusters suggestive of local islands of nuclear envelope (NE) tension. Whereas hyperosmotic shock constricted NPCs analogously to those found in annulate lamellae (AL), depletion of LINC complexes specifically eliminated the modest nuclear surface diameter biases. Therefore, LINC complexes may contribute locally to nuclear envelope tension to toggle NPC diameter between dilated"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.09.18.613744"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11429769"],"repository":["biostudies-literature"],"pubmed_title":["Visualizing nuclear pore complex plasticity with pan-Expansion Microscopy."],"pmcid":["PMC11429769"],"funding_grant_id":["F31 HL158119","R21 AR081661","R01 GM129308","R01 NS122236"],"pubmed_authors":["Coyne AN","Rothstein JD","Morgan KJ","King MC","Carley E","Lusk CP"],"additional_accession":[]},"is_claimable":false,"name":"Visualizing nuclear pore complex plasticity with pan-Expansion Microscopy.","description":"The exploration of cell-type and environmentally-responsive nuclear pore complex (NPC) plasticity requires new, accessible tools. Using pan-Expansion Microscopy (pan-ExM), NPCs were identified by machine learning-facilitated segmentation with resolved cytoplasmic rings (CR), inner rings (IR) and nuclear rings (NR). They exhibited a large range of diameters with a bias for dilated NPCs at the basal nuclear surface in clusters suggestive of local islands of nuclear envelope (NE) tension. Whereas hyperosmotic shock constricted NPCs analogously to those found in annulate lamellae (AL), depletion of LINC complexes specifically eliminated the modest nuclear surface diameter biases. Therefore, LINC complexes may contribute locally to nuclear envelope tension to toggle NPC diameter between dilated","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-04-07T20:19:35.734Z","creation":"2025-04-04T09:59:25.174Z"},"accession":"S-EPMC11429769","cross_references":{"pubmed":["39345637"],"doi":["10.1101/2024.09.18.613744"]}}