biostudies-literatureUnknownTrevisan AJNINDS NIH HHSNCI NIH HHSNeural circuits in the spinal cord are composed of diverse sets of interneurons that play crucial roles in shaping motor output. Despite progress in revealing the cellular architecture of the spinal cord, the extent of cell type heterogeneity within interneuron populations remains unclear. Here, we present a single-nucleus transcriptomic atlas of spinal V1 interneurons across postnatal development. We find that the core molecular taxonomy distinguishing neonatal V1 interneurons perdures into adulthood, suggesting conservation of function across development. Moreover, we identify a key role for En1, a transcription factor that marks the V1 population, in specifying one unique subset of V1^Pou6f2 interneurons. Loss of En1 selectively disrupts the frequency of rhythmic locomotor outbioRxiv : the preprint server for biology2024.09.18.613279https://www.ebi.ac.uk/biostudies/studies/S-EPMC11429899biostudies-literatureThe transcriptomic landscape of spinal V1 interneurons reveals a role for En1 in specific elements of motor output.PMC11429899P30 CA021765R01 NS123116Menon VHan KHinton JMGatto GRamirez CChapman PGabitto MITrevisan AJKulkarni ASKlein IBikoff JBfalseThe transcriptomic landscape of spinal V1 interneurons reveals a role for En1 in specific elements of motor output.Neural circuits in the spinal cord are composed of diverse sets of interneurons that play crucial roles in shaping motor output. Despite progress in revealing the cellular architecture of the spinal cord, the extent of cell type heterogeneity within interneuron populations remains unclear. Here, we present a single-nucleus transcriptomic atlas of spinal V1 interneurons across postnatal development. We find that the core molecular taxonomy distinguishing neonatal V1 interneurons perdures into adulthood, suggesting conservation of function across development. Moreover, we identify a key role for En1, a transcription factor that marks the V1 population, in specifying one unique subset of V1^Pou6f2 interneurons. Loss of En1 selectively disrupts the frequency of rhythmic locomotor out2024-01-01T00:00:00Z2024 Oct2026-05-29T03:17:11.275Z2025-04-04T02:33:16.765ZS-EPMC114298993934558010.1101/2024.09.18.613279