biostudies-literatureUnknown14(9)Tian MStripe rust, caused by <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (<i>Pst</i>), stands out as one of the most devastating epidemics impacting wheat production worldwide. Resistant wheat varieties had swiftly been overcome due to the emergence of new virulent <i>Pst</i> strains. Effectors secreted by <i>Pst</i> interfere with plant immunity, and verification of their biological function is extremely important for controlling wheat stripe rust. In this study, we identified an effector, Pst-18220, from <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (<i>Pst</i>), which was induced during the early infection stage of <i>Pst</i>. Silencing the expression of Pst-18220 through virus-mediated host-induced gene silencing (HIGS) resulted in a decreased number of rust pustules. In <i>NicotiaBiomolecules1092https://www.ebi.ac.uk/biostudies/studies/S-EPMC11430752biostudies-literatureA Putative Effector Pst-18220, from <i>Puccinia striiformis</i> f. sp. <i>tritici</i>, Participates in Rust Pathogenicity and Plant Defense Suppression.PMC11430752Bi XTian MFeng ZZhang ZYuan BLi LWang JXue YZhou JfalseA Putative Effector Pst-18220, from <i>Puccinia striiformis</i> f. sp. <i>tritici</i>, Participates in Rust Pathogenicity and Plant Defense Suppression.Stripe rust, caused by <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (<i>Pst</i>), stands out as one of the most devastating epidemics impacting wheat production worldwide. Resistant wheat varieties had swiftly been overcome due to the emergence of new virulent <i>Pst</i> strains. Effectors secreted by <i>Pst</i> interfere with plant immunity, and verification of their biological function is extremely important for controlling wheat stripe rust. In this study, we identified an effector, Pst-18220, from <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (<i>Pst</i>), which was induced during the early infection stage of <i>Pst</i>. Silencing the expression of Pst-18220 through virus-mediated host-induced gene silencing (HIGS) resulted in a decreased number of rust pustules. In <i>Nicotia2024-01-01T00:00:00Z2024 Aug2026-06-29T03:14:34.132Z2025-04-04T00:34:21.415ZS-EPMC114307523933485810.3390/biom14091092