biostudies-literatureSong BNCRR NIH HHSNIMHD NIH HHSNCI NIH HHS1374https://www.ebi.ac.uk/biostudies/studies/S-EPMC11435809biostudies-literatureUnknown16(9)The productive replication of human immunodeficiency virus type 1 (HIV-1) involves intricate interactions between viral proteins and host cell machinery. However, the contributions of the lysosomal pathways for HIV-1 replication are not fully understood. The goal of this study was to determine the impact of lysosome-targeting compounds on HIV-1 replication and identify the cellular changes that are linked to HIV-1 inhibition using cell culture models of HIV-1 infection. Here, we demonstrate that the treatment of cells with various pharmacological agents known to inhibit lysosomal functions interfere with HIV-1 replication. The vacuolar ATPase (V-ATPase) inhibitor bafilomycin A1 exerted a potent inhibition of HIV-1 replication. Bafilomycin A1 inhibition of HIV-1 was independent of coreceptoVirusesBafilomycin A1 Inhibits HIV-1 Infection by Disrupting Lysosomal Cholesterol Transport.PMC11435809U54 CA163069U54 MD007586S10 RR025497Song BKorolkova OfalseBafilomycin A1 Inhibits HIV-1 Infection by Disrupting Lysosomal Cholesterol Transport.The productive replication of human immunodeficiency virus type 1 (HIV-1) involves intricate interactions between viral proteins and host cell machinery. However, the contributions of the lysosomal pathways for HIV-1 replication are not fully understood. The goal of this study was to determine the impact of lysosome-targeting compounds on HIV-1 replication and identify the cellular changes that are linked to HIV-1 inhibition using cell culture models of HIV-1 infection. Here, we demonstrate that the treatment of cells with various pharmacological agents known to inhibit lysosomal functions interfere with HIV-1 replication. The vacuolar ATPase (V-ATPase) inhibitor bafilomycin A1 exerted a potent inhibition of HIV-1 replication. Bafilomycin A1 inhibition of HIV-1 was independent of corecepto2024-01-01T00:00:00Z2024 Aug2026-07-09T11:11:00.336Z2025-04-04T23:41:46.691ZS-EPMC114358093933985210.3390/v16091374