{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(1)"],"submitter":["Jing X"],"pubmed_abstract":["The nucleosome is one of the hallmarks of eukaryotes, a dynamic platform that supports many critical functions in eukaryotic cells. Here, we engineer the in vivo assembly of the nucleosome core in the model bacterium Escherichia coli. We show that bacterial chromosome DNA and eukaryotic histones can assemble in vivo to form nucleosome complexes with many features resembling those found in eukaryotes. The formation of nucleosomes in E. coli was visualized with atomic force microscopy and using tripartite split green fluorescent protein. Under a condition that moderate histones expression was induced at 1 µM IPTG, the nucleosome-forming bacterium is viable and has sustained growth for at least 110 divisions in longer-term growth experiments. It exhibits stable nucleosome formation, a consist"],"journal":["Nature communications"],"pagination":["8283"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11436726"],"repository":["biostudies-literature"],"pubmed_title":["Creating a bacterium that forms eukaryotic nucleosome core particles."],"pmcid":["PMC11436726"],"pubmed_authors":["Ye BC","Hao P","Chen P","Zhang K","Zhang N","Zhou X","Jing X","Li X","Gong J","Yang S","Wu X","Cai W","Zhao GP"],"additional_accession":[]},"is_claimable":false,"name":"Creating a bacterium that forms eukaryotic nucleosome core particles.","description":"The nucleosome is one of the hallmarks of eukaryotes, a dynamic platform that supports many critical functions in eukaryotic cells. Here, we engineer the in vivo assembly of the nucleosome core in the model bacterium Escherichia coli. We show that bacterial chromosome DNA and eukaryotic histones can assemble in vivo to form nucleosome complexes with many features resembling those found in eukaryotes. The formation of nucleosomes in E. coli was visualized with atomic force microscopy and using tripartite split green fluorescent protein. Under a condition that moderate histones expression was induced at 1 µM IPTG, the nucleosome-forming bacterium is viable and has sustained growth for at least 110 divisions in longer-term growth experiments. It exhibits stable nucleosome formation, a consist","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2026-06-02T04:09:38.163Z","creation":"2025-04-04T12:53:53.103Z"},"accession":"S-EPMC11436726","cross_references":{"pubmed":["39333491"],"doi":["10.1038/s41467-024-52484-2"]}}