{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(1)"],"submitter":["Zhang Y"],"pubmed_abstract":["The 2-oxoglutarate dehydrogenase complex (OGDHc) orchestrates a critical reaction regulating the TCA cycle. Although the structure of each OGDHc subunit has been solved, the architecture of the intact complex and inter-subunit interactions still remain unknown. Here we report the assembly of native, intact OGDHc from Sus scrofa heart tissue using cryo-electron microscopy (cryo-EM), cryo-electron tomography (cryo-ET), and subtomogram averaging (STA) to discern native structures of the whole complex and each subunit. Our cryo-EM analyses revealed the E2o cubic core structure comprising eight homotrimers at 3.3-Å resolution. More importantly, the numbers, positions and orientations of each OGDHc subunit were determined by cryo-ET and the STA structures of the core were resolved at 7.9-Å with "],"journal":["Nature communications"],"pagination":["8407"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11436768"],"repository":["biostudies-literature"],"pubmed_title":["Molecular architecture of the mammalian 2-oxoglutarate dehydrogenase complex."],"pmcid":["PMC11436768"],"pubmed_authors":["Yang Z","Zhang Y","Chen M","Yang M","Zhang M","Chen X","Gao X","Zhang S","Yin J"],"additional_accession":[]},"is_claimable":false,"name":"Molecular architecture of the mammalian 2-oxoglutarate dehydrogenase complex.","description":"The 2-oxoglutarate dehydrogenase complex (OGDHc) orchestrates a critical reaction regulating the TCA cycle. Although the structure of each OGDHc subunit has been solved, the architecture of the intact complex and inter-subunit interactions still remain unknown. Here we report the assembly of native, intact OGDHc from Sus scrofa heart tissue using cryo-electron microscopy (cryo-EM), cryo-electron tomography (cryo-ET), and subtomogram averaging (STA) to discern native structures of the whole complex and each subunit. Our cryo-EM analyses revealed the E2o cubic core structure comprising eight homotrimers at 3.3-Å resolution. More importantly, the numbers, positions and orientations of each OGDHc subunit were determined by cryo-ET and the STA structures of the core were resolved at 7.9-Å with ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2026-06-01T10:05:21.211Z","creation":"2025-04-04T08:22:17.455Z"},"accession":"S-EPMC11436768","cross_references":{"pubmed":["39333186"],"doi":["10.1038/s41467-024-52792-7"]}}