{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tierney BT"],"funding":["U.S. Department of Health & Human Services | NIH | National Institute on Drug Abuse (NIDA)","NCATS NIH HHS","NIDA NIH HHS","NIAID NIH HHS","Kementerian Riset Teknologi Dan Pendidikan Tinggi Republik Indonesia","U.S. Department of Health & Human Services | NIH | National Institute on Drug Abuse","GI Research Foundation WorldQuant"],"pagination":["8386"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11436780"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Wastewater is a geospatially- and temporally-linked microbial fingerprint of a given population, making it a potentially valuable tool for tracking public health across locales and time. Here, we integrate targeted and bulk RNA sequencing (N = 2238 samples) to track the viral, bacterial, and functional content over geospatially distinct areas within Miami Dade County, USA, from 2020-2022. We used targeted amplicon sequencing to track diverse SARS-CoV-2 variants across space and time, and we found a tight correspondence with positive PCR tests from University students and Miami-Dade hospital patients. Additionally, in bulk metatranscriptomic data, we demonstrate that the bacterial content of different wastewater sampling locations serving small population sizes can be used to detect putative, host-derived microorganisms that themselves have known associations with human health and diet. We also detect multiple enteric pathogens (e.g., Norovirus) and characterize viral diversity across sites. Moreover, we observed an enrichment of antimicrobial resistance genes (ARGs) in hospital wastewater; antibiotic-specific ARGs correlated to total prescriptions of those same antibiotics (e.g Ampicillin, Gentamicin). Overall, this effort lays the groundwork for systematic characterization of wastewater that can potentially influence public health decision-making."],"journal":["Nature communications"],"pubmed_title":["Towards geospatially-resolved public-health surveillance via wastewater sequencing."],"pmcid":["PMC11436780"],"funding_grant_id":["R01AI151059","U01 DA053941","R01 AI151059","UM1 TR004556"],"pubmed_authors":["Boone MM","Babler KM","Lamar WE","Mozsary C","Andrews D","Kumar N","Beaver CC","Sharkey ME","Stevenson M","Laine J","Tierney BT","Lucaci AG","Williams SL","Grills GS","Church GM","Currall B","Solle NS","Proszynski J","Yin X","Shukla B","Butler D","Reding B","Mason CE","Wain Hirschberg J","Carattini Y","Amirali A","Schurer SC","Young BG","Comerford S","Solo-Gabriele HM","Tallon JJ","Can Kurt K","Damle N","Vidovic D","Ryon KA","Al Ghalith G","Kobetz E","Foox J"],"additional_accession":[]},"is_claimable":false,"name":"Towards geospatially-resolved public-health surveillance via wastewater sequencing.","description":"Wastewater is a geospatially- and temporally-linked microbial fingerprint of a given population, making it a potentially valuable tool for tracking public health across locales and time. Here, we integrate targeted and bulk RNA sequencing (N = 2238 samples) to track the viral, bacterial, and functional content over geospatially distinct areas within Miami Dade County, USA, from 2020-2022. We used targeted amplicon sequencing to track diverse SARS-CoV-2 variants across space and time, and we found a tight correspondence with positive PCR tests from University students and Miami-Dade hospital patients. Additionally, in bulk metatranscriptomic data, we demonstrate that the bacterial content of different wastewater sampling locations serving small population sizes can be used to detect putative, host-derived microorganisms that themselves have known associations with human health and diet. We also detect multiple enteric pathogens (e.g., Norovirus) and characterize viral diversity across sites. Moreover, we observed an enrichment of antimicrobial resistance genes (ARGs) in hospital wastewater; antibiotic-specific ARGs correlated to total prescriptions of those same antibiotics (e.g Ampicillin, Gentamicin). Overall, this effort lays the groundwork for systematic characterization of wastewater that can potentially influence public health decision-making.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2026-06-13T05:34:57.207Z","creation":"2025-04-04T23:44:11.389Z"},"accession":"S-EPMC11436780","cross_references":{"pubmed":["39333485"],"doi":["10.1038/s41467-024-52427-x"]}}