biostudies-literatureHu DKey Research and Development Program of Hunan ProvinceHealth Research Project of Hunan Provincial Health CommissionHunan Xiangjiang New District (Changsha High-tech Zone) key core technology research project in 2023, and the National Natural Science Foundation of ChinaThe Natural Science Foundation of Changsha(kq2208260), Natural Science Foundation of Hunan ProvincePostgraduate Scientific Research Innovation Project of Hunan Province326https://www.ebi.ac.uk/biostudies/studies/S-EPMC11438047biostudies-literatureUnknown15(1)Cell therapy has emerged as a viable approach for treating damaged organs or tissues, particularly with advancements in stem cell research and regenerative medicine. The innovative technique of cell sheet engineering offers the potential to create a cell-dense lamellar structure that preserves the extracellular matrix (ECM) secreted by cells, along with the cell-matrix and intercellular junctions formed during in vitro cultivation. In recent years, significant progress has been made in developing cell sheet engineering technology. A variety of novel materials and methods were utilized for enzyme-free cell detachment during the cell sheet formation process. The complexity of cell sheet structures increased to meet advanced usage demands. This review aims to provide an overview of the preparStem cell research & therapyThe preparation methods and types of cell sheets engineering.PMC11438047W20242015811015102023SK2069CX201902362019JJ40353Tong PHu DLi JSun YGao CfalseThe preparation methods and types of cell sheets engineering.Cell therapy has emerged as a viable approach for treating damaged organs or tissues, particularly with advancements in stem cell research and regenerative medicine. The innovative technique of cell sheet engineering offers the potential to create a cell-dense lamellar structure that preserves the extracellular matrix (ECM) secreted by cells, along with the cell-matrix and intercellular junctions formed during in vitro cultivation. In recent years, significant progress has been made in developing cell sheet engineering technology. A variety of novel materials and methods were utilized for enzyme-free cell detachment during the cell sheet formation process. The complexity of cell sheet structures increased to meet advanced usage demands. This review aims to provide an overview of the prepar2024-01-01T00:00:00Z2024 Sep2025-04-04T00:51:52.343Z2025-04-04T00:51:52.343ZS-EPMC114380473933440410.1186/s13287-024-03937-4