biostudies-literatureUnknown27(10)Martinez EJDODThe rapid emergence of SARS-CoV-2 variants of concern (VoC) and the threat of future zoonotic sarbecovirus spillover emphasizes the need for broadly protective next-generation vaccines and therapeutics. We utilized SARS-CoV-2 spike ferritin nanoparticle (SpFN), and SARS-CoV-2 receptor binding domain ferritin nanoparticle (RFN) immunogens, in an equine model to elicit hyperimmune sera and evaluated its sarbecovirus neutralization and protection capacity. Immunized animals rapidly elicited sera with the potent neutralization of SARS-CoV-2 VoC, and SARS-CoV-1 pseudoviruses, and potent binding against receptor binding domains from sarbecovirus clades 1b, 1a, 2, 3, and 4. Purified equine polyclonal IgG provided protection against Omicron XBB.1.5 virus in the K18-hACE2 transgenic mouse model. ThiScience110624https://www.ebi.ac.uk/biostudies/studies/S-EPMC11440237biostudies-literatureSARS-CoV-2 ferritin nanoparticle vaccines produce hyperimmune equine sera with broad sarbecovirus activity.PMC11440237Peterson CECollins NDDuso DSankhala RSReagan MGambacurta JLanzer KVasan SChoe MThomas PVGromowski GDKimner JKuklis CKing JDussupt VCorbett CKummer LJoyce MGMayer SVHart TMendez-Rivera LRees PAChang WCSoman SHvizdos JJensen JLKaundinya JFernandes AModjarrad KChen WHCookenham TKrebs SJHajduczki AMichael NLMartinez EJMurzello KfalseSARS-CoV-2 ferritin nanoparticle vaccines produce hyperimmune equine sera with broad sarbecovirus activity.The rapid emergence of SARS-CoV-2 variants of concern (VoC) and the threat of future zoonotic sarbecovirus spillover emphasizes the need for broadly protective next-generation vaccines and therapeutics. We utilized SARS-CoV-2 spike ferritin nanoparticle (SpFN), and SARS-CoV-2 receptor binding domain ferritin nanoparticle (RFN) immunogens, in an equine model to elicit hyperimmune sera and evaluated its sarbecovirus neutralization and protection capacity. Immunized animals rapidly elicited sera with the potent neutralization of SARS-CoV-2 VoC, and SARS-CoV-1 pseudoviruses, and potent binding against receptor binding domains from sarbecovirus clades 1b, 1a, 2, 3, and 4. Purified equine polyclonal IgG provided protection against Omicron XBB.1.5 virus in the K18-hACE2 transgenic mouse model. Th2024-01-01T00:00:00Z2024 Oct2026-07-15T04:15:14.546Z2025-04-04T08:17:33.27ZS-EPMC114402373935119510.1016/j.isci.2024.110624