biostudies-literatureWang DNatural Science Foundation of Fujian ProvinceNational Natural Science Foundation of ChinaThe Joint Funds for the Innovation of Science and Technologye70065https://www.ebi.ac.uk/biostudies/studies/S-EPMC11442333biostudies-literatureUnknown30(10)<h4>Aims</h4>Previous proteomics studies in dysferlinopathy muscle have been limited in scope, often utilizing 2D-electrophoresis and yielding only a small number of differential expression calls. To address this gap, this study aimed to employ high-resolution proteomics to explore the proteomic landscapes of dysferlinopathy and analyze the correlation between muscle pathological changes and alterations in protein expression in muscle biopsies.<h4>Methods</h4>We conducted a comprehensive approach to investigate the proteomic profile and disease-associated changes in the muscle tissue proteome from 15 patients with dysferlinopathy, exhibiting varying degrees of dystrophic pathology, alongside age-matched controls. Our methodology encompasses tandem mass tag (TMT)-labeled liquid chromatograpCNS neuroscience & therapeuticsComprehensive Proteomic Analysis of Dysferlinopathy Unveiling Molecular Mechanisms and Biomarkers Linked to Pathological Progression.PMC114423332020Y912282101471817012422023J01620Zheng YLiu XYWang DChen LLin XZheng FZZeng MHWang NLin YHWang ZQLin FLin MTHe QFChen HZfalseComprehensive Proteomic Analysis of Dysferlinopathy Unveiling Molecular Mechanisms and Biomarkers Linked to Pathological Progression.<h4>Aims</h4>Previous proteomics studies in dysferlinopathy muscle have been limited in scope, often utilizing 2D-electrophoresis and yielding only a small number of differential expression calls. To address this gap, this study aimed to employ high-resolution proteomics to explore the proteomic landscapes of dysferlinopathy and analyze the correlation between muscle pathological changes and alterations in protein expression in muscle biopsies.<h4>Methods</h4>We conducted a comprehensive approach to investigate the proteomic profile and disease-associated changes in the muscle tissue proteome from 15 patients with dysferlinopathy, exhibiting varying degrees of dystrophic pathology, alongside age-matched controls. Our methodology encompasses tandem mass tag (TMT)-labeled liquid chromatograp2024-01-01T00:00:00Z2024 Oct2026-07-15T05:36:15.885Z2025-04-04T19:39:31.992ZS-EPMC114423333935032810.1111/cns.70065