biostudies-literatureWalter JUniversity of Zurichgaae033https://www.ebi.ac.uk/biostudies/studies/S-EPMC11444741biostudies-literatureUnknown30(9)Assisted reproductive technologies are an emerging field in equine reproduction, with species-dependent peculiarities, such as the low success rate of conventional IVF. Here, the 'cumulome' was related to the developmental capacity of its corresponding oocyte. Cumulus-oocyte complexes collected from slaughterhouse ovaries were individually matured, fertilized by ICSI, and cultured. After maturation, the cumulus was collected for proteomics analysis using label-free mass spectrometry (MS)-based protein profiling by nano-HPLC MS/MS and metabolomics analysis by UPLC-nanoESI MS. Overall, a total of 1671 proteins and 612 metabolites were included in the quantifiable 'cumulome'. According to the development of the corresponding oocytes, three groups were compared with each other: not matured (NMMolecular human reproductionMaturational competence of equine oocytes is associated with alterations in their 'cumulome'.PMC11444741FK-13-062Naegeli HGalli CWalter JColleoni SLaczko EBleul ULazzari GFortes CRoschitzki BGrossmann JfalseMaturational competence of equine oocytes is associated with alterations in their 'cumulome'.Assisted reproductive technologies are an emerging field in equine reproduction, with species-dependent peculiarities, such as the low success rate of conventional IVF. Here, the 'cumulome' was related to the developmental capacity of its corresponding oocyte. Cumulus-oocyte complexes collected from slaughterhouse ovaries were individually matured, fertilized by ICSI, and cultured. After maturation, the cumulus was collected for proteomics analysis using label-free mass spectrometry (MS)-based protein profiling by nano-HPLC MS/MS and metabolomics analysis by UPLC-nanoESI MS. Overall, a total of 1671 proteins and 612 metabolites were included in the quantifiable 'cumulome'. According to the development of the corresponding oocytes, three groups were compared with each other: not matured (NM2024-01-01T00:00:00Z2024 Sep2026-06-06T03:22:21.016Z2025-04-07T07:29:24.643ZS-EPMC114447413928833010.1093/molehr/gaae033