biostudies-literatureUnknown15Chen XNatural plant-derived compounds with broad-spectrum antimicrobial activity have become an effective strategy against multidrug-resistant bacteria. The present study was designed to compare the antibacterial activity of six chlorogenic acid (CA) isomers extracted from stevia and investigated the underlying antibacterial mechanisms involved. The results indicated that isochlorogenic acid C (ICAC) exhibited the strongest antibacterial activity against the tested bacteria, especially <i>E. coli</i>, at a 2 mg/mL minimum inhibitory concentration (MIC) and 8 mg/mL minimum bactericidal concentration (MBC). At the MBC, ICAC inhibited 72.66% of the clinical multidrug-resistant strains. Scanning electron microscopy (SEM) revealed that ICAC induced considerable morphological alterations in <i>E. coliFrontiers in microbiology1397906https://www.ebi.ac.uk/biostudies/studies/S-EPMC11445074biostudies-literatureAntibacterial activity and mechanism of Stevia extract against antibiotic-resistant <i>Escherichia coli</i> by interfering with the permeability of the cell wall and the membrane.PMC11445074Cao MZWu LFShang ZXZhu ZZhang JYYi LKBai YBChen XWang WWLi JJXu MLfalseAntibacterial activity and mechanism of Stevia extract against antibiotic-resistant <i>Escherichia coli</i> by interfering with the permeability of the cell wall and the membrane.Natural plant-derived compounds with broad-spectrum antimicrobial activity have become an effective strategy against multidrug-resistant bacteria. The present study was designed to compare the antibacterial activity of six chlorogenic acid (CA) isomers extracted from stevia and investigated the underlying antibacterial mechanisms involved. The results indicated that isochlorogenic acid C (ICAC) exhibited the strongest antibacterial activity against the tested bacteria, especially <i>E. coli</i>, at a 2 mg/mL minimum inhibitory concentration (MIC) and 8 mg/mL minimum bactericidal concentration (MBC). At the MBC, ICAC inhibited 72.66% of the clinical multidrug-resistant strains. Scanning electron microscopy (SEM) revealed that ICAC induced considerable morphological alterations in <i>E. coli2024-01-01T00:00:00Z20242025-04-04T01:01:12.47Z2025-04-04T01:01:12.47ZS-EPMC114450743936032510.3389/fmicb.2024.1397906