{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rubio-Monterde A"],"funding":["Generalitat de Catalunya","Centro de Investigación en Nanociencia y Nanotecnología","Ministerio de Ciencia e Innovación","Agencia Estatal de Investigación","Horizon 2020","Severo Ochoa"],"pagination":["638"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11445331"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["191(10)"],"pubmed_abstract":["Mainly performed within a rapid diagnostic tests company, a lateral flow (LF) system using gold nanoparticles (AuNPs) as transducers is presented able to detect three bacteria of interest, of relevance for antimicrobial resistance (AMR): Clostridioides difficile, methicillin-resistant Staphylococcus aureus (MRSA), and Klebsiella pneumoniae, with a limit of detection of 25 ng/mL of glutamate dehydrogenase (GDH) for C. difficile, 36 ng/mL of penicillin-binding protein 2a (PBP2a) for MRSA, and 4 × 10<sup>6</sup> CFU/mL for K. pneumoniae. The system showed good results with bacteria culture samples, is user-friendly, and suitable for rapid testing, as the results are obtained within 15 min."],"journal":["Mikrochimica acta"],"pubmed_title":["Lateral flow immunoassay for simultaneous detection of C. difficile, MRSA, and K. pneumoniae."],"pmcid":["PMC11445331"],"funding_grant_id":["10.13039/501100011033","688878","PTQ2018-010113","DIN2018-010115","CEX2021-001214-S","2021 SGR 01464"],"pubmed_authors":["Rivas L","Quesada-Gonzalez D","Merkoci A","Gallegos M","Rubio-Monterde A"],"additional_accession":[]},"is_claimable":false,"name":"Lateral flow immunoassay for simultaneous detection of C. difficile, MRSA, and K. pneumoniae.","description":"Mainly performed within a rapid diagnostic tests company, a lateral flow (LF) system using gold nanoparticles (AuNPs) as transducers is presented able to detect three bacteria of interest, of relevance for antimicrobial resistance (AMR): Clostridioides difficile, methicillin-resistant Staphylococcus aureus (MRSA), and Klebsiella pneumoniae, with a limit of detection of 25 ng/mL of glutamate dehydrogenase (GDH) for C. difficile, 36 ng/mL of penicillin-binding protein 2a (PBP2a) for MRSA, and 4 × 10<sup>6</sup> CFU/mL for K. pneumoniae. The system showed good results with bacteria culture samples, is user-friendly, and suitable for rapid testing, as the results are obtained within 15 min.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2025-04-05T08:58:21.299Z","creation":"2025-04-05T08:58:21.299Z"},"accession":"S-EPMC11445331","cross_references":{"pubmed":["39352552"],"doi":["10.1007/s00604-024-06701-w"]}}