biostudies-literatureRubio-Monterde AGeneralitat de CatalunyaCentro de Investigación en Nanociencia y NanotecnologíaMinisterio de Ciencia e InnovaciónAgencia Estatal de InvestigaciónHorizon 2020Severo Ochoa638https://www.ebi.ac.uk/biostudies/studies/S-EPMC11445331biostudies-literatureUnknown191(10)Mainly performed within a rapid diagnostic tests company, a lateral flow (LF) system using gold nanoparticles (AuNPs) as transducers is presented able to detect three bacteria of interest, of relevance for antimicrobial resistance (AMR): Clostridioides difficile, methicillin-resistant Staphylococcus aureus (MRSA), and Klebsiella pneumoniae, with a limit of detection of 25 ng/mL of glutamate dehydrogenase (GDH) for C. difficile, 36 ng/mL of penicillin-binding protein 2a (PBP2a) for MRSA, and 4 × 10⁶ CFU/mL for K. pneumoniae. The system showed good results with bacteria culture samples, is user-friendly, and suitable for rapid testing, as the results are obtained within 15 min.Mikrochimica actaLateral flow immunoassay for simultaneous detection of C. difficile, MRSA, and K. pneumoniae.PMC1144533110.13039/501100011033688878PTQ2018-010113DIN2018-010115CEX2021-001214-S2021 SGR 01464Rivas LQuesada-Gonzalez DMerkoci AGallegos MRubio-Monterde AfalseLateral flow immunoassay for simultaneous detection of C. difficile, MRSA, and K. pneumoniae.Mainly performed within a rapid diagnostic tests company, a lateral flow (LF) system using gold nanoparticles (AuNPs) as transducers is presented able to detect three bacteria of interest, of relevance for antimicrobial resistance (AMR): Clostridioides difficile, methicillin-resistant Staphylococcus aureus (MRSA), and Klebsiella pneumoniae, with a limit of detection of 25 ng/mL of glutamate dehydrogenase (GDH) for C. difficile, 36 ng/mL of penicillin-binding protein 2a (PBP2a) for MRSA, and 4 × 10⁶ CFU/mL for K. pneumoniae. The system showed good results with bacteria culture samples, is user-friendly, and suitable for rapid testing, as the results are obtained within 15 min.2024-01-01T00:00:00Z2024 Oct2025-04-05T08:58:21.299Z2025-04-05T08:58:21.299ZS-EPMC114453313935255210.1007/s00604-024-06701-w