biostudies-literatureNava MNational Institute of Allergy and Infectious DiseasesNIAID NIH HHSNational Institute of General Medical SciencesAir Force Office of Scientific ResearchNIGMS NIH HHS2440-2448https://www.ebi.ac.uk/biostudies/studies/S-EPMC11447908biostudies-literatureUnknown63(19)Nonvesicular lipid trafficking pathways are an important process in every domain of life. The mechanisms of these processes are poorly understood in part due to the difficulty in kinetic characterization. One important class of glycolipids, lipopolysaccharides (LPS), are the primary lipidic component of the outer membrane of Gram-negative bacteria. LPS are synthesized in the inner membrane and then trafficked to the cell surface by the <b>l</b>ipo<b>p</b>olysaccharide <b>t</b>ransport proteins, <u>Lpt</u>B₂FGCADE. By characterizing the interaction of a fluorescent probe and LPS, we establish a quantitative assay to monitor the flux of LPS between proteoliposomes on the time scale of seconds. We then incorporate photocaged ATP into this system, which allows for light-based controBiochemistryDetermination of Initial Rates of Lipopolysaccharide Transport.PMC11447908FA9550-19-1-0246R01 GM047274R01 AI149778R01 AI081059Nocera DGTaylor RJRowe SJKahne DNava MfalseDetermination of Initial Rates of Lipopolysaccharide Transport.Nonvesicular lipid trafficking pathways are an important process in every domain of life. The mechanisms of these processes are poorly understood in part due to the difficulty in kinetic characterization. One important class of glycolipids, lipopolysaccharides (LPS), are the primary lipidic component of the outer membrane of Gram-negative bacteria. LPS are synthesized in the inner membrane and then trafficked to the cell surface by the <b>l</b>ipo<b>p</b>olysaccharide <b>t</b>ransport proteins, <u>Lpt</u>B₂FGCADE. By characterizing the interaction of a fluorescent probe and LPS, we establish a quantitative assay to monitor the flux of LPS between proteoliposomes on the time scale of seconds. We then incorporate photocaged ATP into this system, which allows for light-based contro2024-01-01T00:00:00Z2024 Oct2026-06-01T09:05:28.468Z2025-04-06T16:11:25.911ZS-EPMC114479083926432810.1021/acs.biochem.4c00379