{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Zhang Y"],"funding":["Chan Zuckerberg Biohub","NCI NIH HHS","National Institutes of Health","Japan Society for the Promotion of Science"],"pubmed_abstract":["Extracellular signal-regulated kinase (ERK) signaling is essential to regulated cell behaviors, including cell proliferation, differentiation, and apoptosis. The influence of cell-cell contacts on ERK signaling is central to epithelial cells, yet few studies have sought to understand the same in cancer cells, particularly with single-cell resolution. To acquire same-cell measurements of both phenotypic (cell-contact state) and targeted-protein (ERK phosphorylation) profiles, we prepend high-content, whole-cell imaging prior to end-point cellular-resolution Western blot analyses for each of hundreds of individual HeLa cancer cells cultured on that same chip, which we call <i>contact</i>Blot. By indexing the phosphorylation level of ERK in each cell or cell cluster to the imaged cell-contact"],"journal":["Analytical chemistry"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11447967"],"repository":["biostudies-literature"],"pubmed_title":["&lt;i&gt;Contact&lt;/i&gt;Blot: Microfluidic Control and Measurement of the Cell-Cell Contact State to Assess Contact-Inhibited ERK Signaling."],"pmcid":["PMC11447967"],"funding_grant_id":["R01CA203018","R33 CA225296","JP22H02761","JP18H02569","R01 CA203018","R33CA225296","JP15KK0297"],"pubmed_authors":["Herr AE","Zhang Y","Naguro I","Ryuno H"],"additional_accession":[]},"is_claimable":false,"name":"&lt;i&gt;Contact&lt;/i&gt;Blot: Microfluidic Control and Measurement of the Cell-Cell Contact State to Assess Contact-Inhibited ERK Signaling.","description":"Extracellular signal-regulated kinase (ERK) signaling is essential to regulated cell behaviors, including cell proliferation, differentiation, and apoptosis. The influence of cell-cell contacts on ERK signaling is central to epithelial cells, yet few studies have sought to understand the same in cancer cells, particularly with single-cell resolution. To acquire same-cell measurements of both phenotypic (cell-contact state) and targeted-protein (ERK phosphorylation) profiles, we prepend high-content, whole-cell imaging prior to end-point cellular-resolution Western blot analyses for each of hundreds of individual HeLa cancer cells cultured on that same chip, which we call <i>contact</i>Blot. By indexing the phosphorylation level of ERK in each cell or cell cluster to the imaged cell-contact","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2025-04-26T10:24:15.944Z","creation":"2025-04-06T13:23:04.313Z"},"accession":"S-EPMC11447967","cross_references":{"pubmed":["39254112"],"doi":["10.1021/acs.analchem.4c02936"]}}