biostudies-literatureUnknownZhang YChan Zuckerberg BiohubNCI NIH HHSNational Institutes of HealthJapan Society for the Promotion of ScienceExtracellular signal-regulated kinase (ERK) signaling is essential to regulated cell behaviors, including cell proliferation, differentiation, and apoptosis. The influence of cell-cell contacts on ERK signaling is central to epithelial cells, yet few studies have sought to understand the same in cancer cells, particularly with single-cell resolution. To acquire same-cell measurements of both phenotypic (cell-contact state) and targeted-protein (ERK phosphorylation) profiles, we prepend high-content, whole-cell imaging prior to end-point cellular-resolution Western blot analyses for each of hundreds of individual HeLa cancer cells cultured on that same chip, which we call <i>contact</i>Blot. By indexing the phosphorylation level of ERK in each cell or cell cluster to the imaged cell-contactAnalytical chemistryhttps://www.ebi.ac.uk/biostudies/studies/S-EPMC11447967biostudies-literature&lt;i&gt;Contact&lt;/i&gt;Blot: Microfluidic Control and Measurement of the Cell-Cell Contact State to Assess Contact-Inhibited ERK Signaling.PMC11447967R01CA203018R33 CA225296JP22H02761JP18H02569R01 CA203018R33CA225296JP15KK0297Herr AEZhang YNaguro IRyuno Hfalse&lt;i&gt;Contact&lt;/i&gt;Blot: Microfluidic Control and Measurement of the Cell-Cell Contact State to Assess Contact-Inhibited ERK Signaling.Extracellular signal-regulated kinase (ERK) signaling is essential to regulated cell behaviors, including cell proliferation, differentiation, and apoptosis. The influence of cell-cell contacts on ERK signaling is central to epithelial cells, yet few studies have sought to understand the same in cancer cells, particularly with single-cell resolution. To acquire same-cell measurements of both phenotypic (cell-contact state) and targeted-protein (ERK phosphorylation) profiles, we prepend high-content, whole-cell imaging prior to end-point cellular-resolution Western blot analyses for each of hundreds of individual HeLa cancer cells cultured on that same chip, which we call <i>contact</i>Blot. By indexing the phosphorylation level of ERK in each cell or cell cluster to the imaged cell-contact2024-01-01T00:00:00Z2024 Sep2025-04-26T10:24:15.944Z2025-04-06T13:23:04.313ZS-EPMC114479673925411210.1021/acs.analchem.4c02936