biostudies-literatureLuo LNatural Science Project of Guizhou UniversityScience and Technology Project of Guizhou ProvinceNatural Science Project of Guizhou University (Special Post)Qianxinan Branch of Guizhou Tobacco CompanyNational Natural Science Foundation of ChinaAnshun Branch of Guizhou Tobacco Companye0105324https://www.ebi.ac.uk/biostudies/studies/S-EPMC11448085biostudies-literatureUnknown12(10)<i>Cordyceps militaris</i> infects insects and forms sclerotia within the insect remains, establishing insect-microbe complexes. Here, <i>C</i>. <i>militaris</i> sclerotia samples from a single location in China over a 5-year period were subjected to high-throughput DNA sequencing, and the core microbes (which were stably enriched in the sclerotia over the 5 years) were identified. Next, seven bacterial strains were isolated from the <i>C. militaris</i> sclerotia, their biochemical characteristics were assessed, and they were co-cultured with <i>C. militaris</i> to study their effects on <i>C. militaris</i> metabolite production and biomass. Furthermore, the effects of NH₄, NO₃, and peptone media on <i>C. militaris</i> were compared. The results showed that <i>RhodocoMicrobiology spectrumCore microbes in <i>Cordyceps militaris</i> sclerotia and their nitrogen metabolism-related ecological functions.PMC11448085320600382020XM14Zhou Yan Si [2022] No 6 2022-04Gui Da Te Gang He Zi [2022] 51Qian Ke He Foundation [2020] 1Z009Zou XGuan JLuo LYao MFei GQu JDai FZhou YXu ZXue YfalseCore microbes in <i>Cordyceps militaris</i> sclerotia and their nitrogen metabolism-related ecological functions.<i>Cordyceps militaris</i> infects insects and forms sclerotia within the insect remains, establishing insect-microbe complexes. Here, <i>C</i>. <i>militaris</i> sclerotia samples from a single location in China over a 5-year period were subjected to high-throughput DNA sequencing, and the core microbes (which were stably enriched in the sclerotia over the 5 years) were identified. Next, seven bacterial strains were isolated from the <i>C. militaris</i> sclerotia, their biochemical characteristics were assessed, and they were co-cultured with <i>C. militaris</i> to study their effects on <i>C. militaris</i> metabolite production and biomass. Furthermore, the effects of NH₄, NO₃, and peptone media on <i>C. militaris</i> were compared. The results showed that <i>Rhodoco2024-01-01T00:00:00Z2024 Oct2026-06-16T03:11:14.659Z2025-04-04T12:54:08.884ZS-EPMC114480853916254110.1128/spectrum.01053-24