biostudies-literatureMaguvu TECalifornia Cherry Boarde0132424https://www.ebi.ac.uk/biostudies/studies/S-EPMC11448091biostudies-literatureUnknown12(10)To gain insights into the diversity of <i>Pseudomonas syringae</i> sensu lato affecting sweet cherry in California, we sequenced and analyzed the phylogenomic and genomic architecture of 86 fluorescent pseudomonads isolated from symptomatic and asymptomatic cherry tissues. Fifty-eight isolates were phylogenetically placed within the <i>P. syringae</i> species complex and taxonomically classified into five genomospecies: <i>P. syringae</i> pv. <i>syringae</i>, <i>P. syringae</i>, <i>Pseudomonas cerasi</i>, <i>Pseudomonas viridiflava</i>, and <i>A</i>. We annotated components of the type III secretion system and phytotoxin-encoding genes and correlated the data with pathogenicity phenotypes. Intact probable regulatory protein HrpR was annotated in the genomic sequences of all isolates of <i>Microbiology spectrumPathogenicity, phylogenomic, and comparative genomic study of <i>Pseudomonas syringae</i> sensu lato affecting sweet cherry in California.PMC1144809123-CCB5400-0224-CCB5400-02Dardani GShipley ENouri MTYaghmour MAMaguvu TETrouillas FPFrias RJHernandez-Rosas AIfalsePathogenicity, phylogenomic, and comparative genomic study of <i>Pseudomonas syringae</i> sensu lato affecting sweet cherry in California.To gain insights into the diversity of <i>Pseudomonas syringae</i> sensu lato affecting sweet cherry in California, we sequenced and analyzed the phylogenomic and genomic architecture of 86 fluorescent pseudomonads isolated from symptomatic and asymptomatic cherry tissues. Fifty-eight isolates were phylogenetically placed within the <i>P. syringae</i> species complex and taxonomically classified into five genomospecies: <i>P. syringae</i> pv. <i>syringae</i>, <i>P. syringae</i>, <i>Pseudomonas cerasi</i>, <i>Pseudomonas viridiflava</i>, and <i>A</i>. We annotated components of the type III secretion system and phytotoxin-encoding genes and correlated the data with pathogenicity phenotypes. Intact probable regulatory protein HrpR was annotated in the genomic sequences of all isolates of <i>2024-01-01T00:00:00Z2024 Oct2025-04-05T08:58:04.281Z2025-04-05T08:58:04.281ZS-EPMC114480913922547310.1128/spectrum.01324-24