biostudies-literatureUnknown15(1)Torta FIn this community effort, we compare measurements between 34 laboratories from 19 countries, utilizing mixtures of labelled authentic synthetic standards, to quantify by mass spectrometry four clinically used ceramide species in the NIST (National Institute of Standards and Technology) human blood plasma Standard Reference Material (SRM) 1950, as well as a set of candidate plasma reference materials (RM 8231). Participants either utilized a provided validated method and/or their method of choice. Mean concentration values, and intra- and inter-laboratory coefficients of variation (CV) were calculated using single-point and multi-point calibrations, respectively. These results are the most precise (intra-laboratory CVs ≤ 4.2%) and concordant (inter-laboratory CVs < 14%) community-derived abNature communications8562https://www.ebi.ac.uk/biostudies/studies/S-EPMC11449902biostudies-literatureConcordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards.PMC11449902Harms ALee JCRamanathan AMoon MHSaigusa DAhrends RSousa BCCheca AMath RGHMartinefski MRWheelock CEHuynh KCala MPSingh MCinel MGhorasaini MLange MWolrab DChocholouskova MShui GTsugawa HBertrand-Michel JChen MComan CKofeler HCO'Donnell VBLippa KXu GBamba TSchwartz-Zimmermann HHu CMonge METournadre AConnell LThorsteinsdottir MMayor SXu THoffmann NChu-Van ELam SMCamacho-Munoz DHaid MWakelam MOFedorova MIvanisevic JColsch BGallart-Ayala HBurla BTyrrell VJDickens AMBennett SALSchock TBTomiyasu NEkroos KOresic MHolcapek MManzi MTakahashi MRiols FIzumi YNguyen-Tran THulsmeier AJLopez-Clavijo AFvan der Gugten GBowden JAArita MMuralidharan SAlecu IWenk MRHoring MHornemann TJones CMGuan XLNicolaou AGiera MBrugger BHankemeier TMeikle PJTorta FLiebisch GEiriksson FFfalseConcordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards.In this community effort, we compare measurements between 34 laboratories from 19 countries, utilizing mixtures of labelled authentic synthetic standards, to quantify by mass spectrometry four clinically used ceramide species in the NIST (National Institute of Standards and Technology) human blood plasma Standard Reference Material (SRM) 1950, as well as a set of candidate plasma reference materials (RM 8231). Participants either utilized a provided validated method and/or their method of choice. Mean concentration values, and intra- and inter-laboratory coefficients of variation (CV) were calculated using single-point and multi-point calibrations, respectively. These results are the most precise (intra-laboratory CVs ≤ 4.2%) and concordant (inter-laboratory CVs < 14%) community-derived ab2024-01-01T00:00:00Z2024 Oct2025-04-05T08:57:20.174Z2025-04-05T08:57:20.174ZS-EPMC114499023936284310.1038/s41467-024-52087-x