biostudies-literatureBautista-Bautista GInstituto Nacional de Perinatología "Isidro Espinosa de los Reyes", Ciudad de México, México.645https://www.ebi.ac.uk/biostudies/studies/S-EPMC11451097biostudies-literatureUnknown24(1)<h4>Background</h4>Escherichia coli (E. coli) is one of the main bacteria associated with preterm premature rupture of membranes by increasing pro-matrix metalloproteinase 9 (proMMP-9) and degradation of type IV collagen in human feto-maternal interface (HFMi). proMMP-9 is regulated by progesterone (P4) but it is unclear whether P4 inhibits proMMP in human maternal decidual (MDec). This study aimed to determine a role of P4 on proMMP-2 and - 9 and type IV collagen induced by E. coli infection in MDec.<h4>Methods</h4>Nine HFMi were mounted in a Transwell system. MDec was stimulated with P4 or E. coli for 3-, 6-, or 24-hours. proMMP-2, -9 and type IV collagen were assessed.<h4>Results</h4>Gelatin zymography revealed an increase in proMMP-9 after 3, 6, and 24 h of stimulating MDec with E. colBMC pregnancy and childbirthEscherichia coli induced matrix metalloproteinase-9 activity and type IV collagen degradation is regulated by progesterone in human maternal decidual.PMC114510972017-2-82Lara-Pereyra IFlores-Herrera HVilleda-Gabriel GAcuna-Gonzalez RJBautista-Bautista GDiaz-Ruiz OPalafox-Vargas MLGarcia-Lopez GSalguero-Zacarias SOsorio-Caballero MfalseEscherichia coli induced matrix metalloproteinase-9 activity and type IV collagen degradation is regulated by progesterone in human maternal decidual.<h4>Background</h4>Escherichia coli (E. coli) is one of the main bacteria associated with preterm premature rupture of membranes by increasing pro-matrix metalloproteinase 9 (proMMP-9) and degradation of type IV collagen in human feto-maternal interface (HFMi). proMMP-9 is regulated by progesterone (P4) but it is unclear whether P4 inhibits proMMP in human maternal decidual (MDec). This study aimed to determine a role of P4 on proMMP-2 and - 9 and type IV collagen induced by E. coli infection in MDec.<h4>Methods</h4>Nine HFMi were mounted in a Transwell system. MDec was stimulated with P4 or E. coli for 3-, 6-, or 24-hours. proMMP-2, -9 and type IV collagen were assessed.<h4>Results</h4>Gelatin zymography revealed an increase in proMMP-9 after 3, 6, and 24 h of stimulating MDec with E. col2024-01-01T00:00:00Z2024 Oct2026-06-07T08:12:27.356Z2025-04-04T02:00:46.846ZS-EPMC114510973936734010.1186/s12884-024-06847-8