{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Butters A"],"funding":["Beef Cattle Research Council (BCRC)","Beef Cattle Research Council","Canadian Government | Natural Sciences and Engineering Research Council of Canada (NSERC)","Results Driven Agricultural Research","Alberta Beef Producers","Alberta Beef Producers (ABP)","Canadian Government | Natural Sciences and Engineering Research Council of Canada"],"pagination":["e0053224"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11451601"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(10)"],"pubmed_abstract":["Colistin resistance in <i>Escherichia coli</i> is of public health significance for its use to treat multidrug-resistant Gram-negative infections. Amino acid variations in PmrB have been implicated in colistin resistance in <i>E. coli</i>. In this cross-sectional study, 288 generic <i>E. coli</i> isolates from surveillance of broiler chicken and feedlot cattle feces, retail meat, wastewater, and well water were whole-genome sequenced. Phylogroup designation and screening for two amino acid substitutions in PmrB putatively linked to colistin resistance (Y358N, E123D) were performed <i>in silico</i>. Three additional data sets of publicly available <i>E. coli</i> assemblies were similarly scrutinized: (i) <i>E. coli</i> isolates from studies identifying the Y358N or E123D substitutions, (ii)"],"journal":["Microbiology spectrum"],"pubmed_title":["PmrB Y358N, E123D amino acid substitutions are not associated with colistin resistance but with phylogeny in <i>Escherichia coli</i>."],"pmcid":["PMC11451601"],"funding_grant_id":["RGPIN/05307-2019","ANH.29.20","2021R098R","2021R098RC"],"pubmed_authors":["Checkley SL","Liljebjelke K","Butters A","Jovel J","Waldner C","Gow S"],"additional_accession":[]},"is_claimable":false,"name":"PmrB Y358N, E123D amino acid substitutions are not associated with colistin resistance but with phylogeny in <i>Escherichia coli</i>.","description":"Colistin resistance in <i>Escherichia coli</i> is of public health significance for its use to treat multidrug-resistant Gram-negative infections. Amino acid variations in PmrB have been implicated in colistin resistance in <i>E. coli</i>. In this cross-sectional study, 288 generic <i>E. coli</i> isolates from surveillance of broiler chicken and feedlot cattle feces, retail meat, wastewater, and well water were whole-genome sequenced. Phylogroup designation and screening for two amino acid substitutions in PmrB putatively linked to colistin resistance (Y358N, E123D) were performed <i>in silico</i>. Three additional data sets of publicly available <i>E. coli</i> assemblies were similarly scrutinized: (i) <i>E. coli</i> isolates from studies identifying the Y358N or E123D substitutions, (ii)","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-06-08T03:31:24.137Z","creation":"2025-04-04T00:34:04.761Z"},"accession":"S-EPMC11451601","cross_references":{"pubmed":["39162501"],"doi":["10.1128/spectrum.00532-24"]}}