{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Srivastava A"],"funding":["Indiana Alzheimer’s Disease Research Center","Natural Sciences and Engineering Research Council of Canada","Intramural NIH HHS","NIA NIH HHS","Division of Intramural Research, National Institute of Allergy and Infectious Diseases","National Institute on Aging","Interdisciplinary Theoretical and Mathematical Sciences programme at RIKEN"],"pagination":["e1012554"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11451978"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(9)"],"pubmed_abstract":["Disease associated pathological aggregates of alpha-synuclein (αSynD) exhibit prion-like spreading in synucleinopathies such as Parkinson's disease (PD) and dementia with Lewy bodies (DLB). Seed amplification assays (SAAs) such as real-time quaking-induced conversion (RT-QuIC) have shown high diagnostic sensitivity and specificity for detecting proteopathic αSynD seeds in a variety of biospecimens from PD and DLB patients. However, the extent to which relative proteopathic seed concentrations are useful as indices of a patient's disease stage or prognosis remains unresolved. One feature of current SAAs that complicates attempts to correlate SAA results with patients' clinical and other laboratory findings is their quantitative imprecision, which has typically been limited to discriminating"],"journal":["PLoS pathogens"],"pubmed_title":["Enhanced quantitation of pathological α-synuclein in patient biospecimens by RT-QuIC seed amplification assays."],"pmcid":["PMC11451978"],"funding_grant_id":["P30 AG072976","Discovery Grant 2022-03744","ZIA AI001086","ZIAAI001086","AGO5131"],"pubmed_authors":["Fernandez M","Wang Q","Compta Y","Zanusso G","Ghetti B","Orru CD","Zou WQ","Beauchemin CAA","Srivastava A","Caughey B"],"additional_accession":[]},"is_claimable":false,"name":"Enhanced quantitation of pathological α-synuclein in patient biospecimens by RT-QuIC seed amplification assays.","description":"Disease associated pathological aggregates of alpha-synuclein (αSynD) exhibit prion-like spreading in synucleinopathies such as Parkinson's disease (PD) and dementia with Lewy bodies (DLB). Seed amplification assays (SAAs) such as real-time quaking-induced conversion (RT-QuIC) have shown high diagnostic sensitivity and specificity for detecting proteopathic αSynD seeds in a variety of biospecimens from PD and DLB patients. However, the extent to which relative proteopathic seed concentrations are useful as indices of a patient's disease stage or prognosis remains unresolved. One feature of current SAAs that complicates attempts to correlate SAA results with patients' clinical and other laboratory findings is their quantitative imprecision, which has typically been limited to discriminating","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2026-06-13T05:33:32.53Z","creation":"2026-06-13T03:09:12.062Z"},"accession":"S-EPMC11451978","cross_references":{"pubmed":["39302978"],"doi":["10.1371/journal.ppat.1012554"]}}