{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["23(1)"],"submitter":["Bechtel A"],"funding":["Universität Hohenheim"],"pubmed_abstract":["<h4>Background</h4>The β-galactosidase from Paenibacillus wynnii (β-gal-Pw) is a promising candidate for lactose hydrolysis in milk and dairy products, as it has a higher affinity for the substrate lactose (low K<sub>M</sub> value) compared to industrially used β-galactosidases and is not inhibited by the hydrolysis-generated product D-galactose. However, β-gal-Pw must firstly be produced cost-effectively for any potential industrial application. Accordingly, the yeast Komagataella phaffii was chosen to investigate its feasibility to recombinantly produce β-gal-Pw since it is approved for the regulated production of food enzymes. The aim of this study was to find the most suitable way to produce the β-gal-Pw in K. phaffii either extracellularly or intracellularly.<h4>Results</h4>Firstly, 1"],"journal":["Microbial cell factories"],"pagination":["263"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11452983"],"repository":["biostudies-literature"],"pubmed_title":["Recombinant production of Paenibacillus wynnii β-galactosidase with Komagataella phaffii."],"pmcid":["PMC11452983"],"pubmed_authors":["Hetzel F","Seitl I","Fischer L","Bechtel A","Keutgen M","Pross E"],"additional_accession":[]},"is_claimable":false,"name":"Recombinant production of Paenibacillus wynnii β-galactosidase with Komagataella phaffii.","description":"<h4>Background</h4>The β-galactosidase from Paenibacillus wynnii (β-gal-Pw) is a promising candidate for lactose hydrolysis in milk and dairy products, as it has a higher affinity for the substrate lactose (low K<sub>M</sub> value) compared to industrially used β-galactosidases and is not inhibited by the hydrolysis-generated product D-galactose. However, β-gal-Pw must firstly be produced cost-effectively for any potential industrial application. Accordingly, the yeast Komagataella phaffii was chosen to investigate its feasibility to recombinantly produce β-gal-Pw since it is approved for the regulated production of food enzymes. The aim of this study was to find the most suitable way to produce the β-gal-Pw in K. phaffii either extracellularly or intracellularly.<h4>Results</h4>Firstly, 1","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-06-03T03:43:19.835Z","creation":"2025-04-06T22:28:35.785Z"},"accession":"S-EPMC11452983","cross_references":{"pubmed":["39367390"],"doi":["10.1186/s12934-024-02544-5"]}}