biostudies-literatureUnknown23(1)Bechtel AUniversität Hohenheim<h4>Background</h4>The β-galactosidase from Paenibacillus wynnii (β-gal-Pw) is a promising candidate for lactose hydrolysis in milk and dairy products, as it has a higher affinity for the substrate lactose (low K_M value) compared to industrially used β-galactosidases and is not inhibited by the hydrolysis-generated product D-galactose. However, β-gal-Pw must firstly be produced cost-effectively for any potential industrial application. Accordingly, the yeast Komagataella phaffii was chosen to investigate its feasibility to recombinantly produce β-gal-Pw since it is approved for the regulated production of food enzymes. The aim of this study was to find the most suitable way to produce the β-gal-Pw in K. phaffii either extracellularly or intracellularly.<h4>Results</h4>Firstly, 1Microbial cell factories263https://www.ebi.ac.uk/biostudies/studies/S-EPMC11452983biostudies-literatureRecombinant production of Paenibacillus wynnii β-galactosidase with Komagataella phaffii.PMC11452983Hetzel FSeitl IFischer LBechtel AKeutgen MPross EfalseRecombinant production of Paenibacillus wynnii β-galactosidase with Komagataella phaffii.<h4>Background</h4>The β-galactosidase from Paenibacillus wynnii (β-gal-Pw) is a promising candidate for lactose hydrolysis in milk and dairy products, as it has a higher affinity for the substrate lactose (low K_M value) compared to industrially used β-galactosidases and is not inhibited by the hydrolysis-generated product D-galactose. However, β-gal-Pw must firstly be produced cost-effectively for any potential industrial application. Accordingly, the yeast Komagataella phaffii was chosen to investigate its feasibility to recombinantly produce β-gal-Pw since it is approved for the regulated production of food enzymes. The aim of this study was to find the most suitable way to produce the β-gal-Pw in K. phaffii either extracellularly or intracellularly.<h4>Results</h4>Firstly, 12024-01-01T00:00:00Z2024 Oct2026-06-03T03:43:19.835Z2025-04-06T22:28:35.785ZS-EPMC114529833936739010.1186/s12934-024-02544-5