biostudies-literatureMonier MEuropean Research Council"Investments for the Future" program, FranceLabex "Who AM I?"Centre National de la Recherche ScientifiqueMinistère de l'Education Nationale, de la Recherche et de la Technologie, France23249https://www.ebi.ac.uk/biostudies/studies/S-EPMC11456580biostudies-literatureUnknown14(1)Drosophila glue, a bioadhesive produced by fly larvae to attach themselves to a substrate for several days, has recently gained attention for its peculiar adhesive and mechanical properties. Although Drosophila glue production was described more than 50 years ago, a general survey of the adhesive and mechanical properties of this proteinaceous gel across Drosophila species is lacking. To measure adhesion, we present here a protocol that is robust to variations in protocol parameters, pupal age and calculation methods. We find that the glue, which covers the entire pupal surface, increases the animal rigidity and plasticity when bound to a glass slide. Our survey of pupal adhesion in 25 Drosophilidae species reveals a wide range of phenotypes, from species that produce no or little glue andScientific reportsAdhesive and mechanical properties of the glue produced by 25 Drosophila species.PMC11456580Université Paris Cité, Idex ANR-18- IDEX-0001PhD FellowshipERC Starting grant - FP7/2007-2013 Grant Agreement No. 337579MITI interdisciplinary action "Défi Adaptation du vivant à son environnement"ANR-11-LABX- 0071Narasimha SMevel LGraner FLorenzi JNPetit RCourtier-Orgogozo VMonier MBorne FContremoulins VEl Hachem YfalseAdhesive and mechanical properties of the glue produced by 25 Drosophila species.Drosophila glue, a bioadhesive produced by fly larvae to attach themselves to a substrate for several days, has recently gained attention for its peculiar adhesive and mechanical properties. Although Drosophila glue production was described more than 50 years ago, a general survey of the adhesive and mechanical properties of this proteinaceous gel across Drosophila species is lacking. To measure adhesion, we present here a protocol that is robust to variations in protocol parameters, pupal age and calculation methods. We find that the glue, which covers the entire pupal surface, increases the animal rigidity and plasticity when bound to a glass slide. Our survey of pupal adhesion in 25 Drosophilidae species reveals a wide range of phenotypes, from species that produce no or little glue and2024-01-01T00:00:00Z2024 Oct2026-06-09T07:18:14.228Z2025-04-07T02:52:48.12ZS-EPMC114565803937042610.1038/s41598-024-74358-9