{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(17)"],"submitter":["Athanasopoulou K"],"pubmed_abstract":["<b>Background:</b> N6-methyladenosine (m6A), a prevalent mRNA modification, is dynamically regulated by methyltransferases, including METTL3 and METTL14.<b>Materials & methods:</b> In the current study, we employed a custom hybrid-seq method to identify novel <i>METTL3</i>/<i>14</i> transcripts, explore their protein-coding capacities and predict the putative role of the METTL isoforms.<b>Results:</b> Demultiplexing of the hybrid-seq barcoded datasets unraveled the expression patterns of the newly identified mRNAs in major malignancies as well as in non-malignant cells, providing a deeper understanding of the methylation pathways. Open reading frame query revealed novel METTL3/14 isoforms, broadening our perspective for the structural diversity within METTL family.<b>Conclusion:</b> Our fi"],"journal":["Epigenomics"],"pagination":["1159-1174"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11457658"],"repository":["biostudies-literature"],"pubmed_title":["New insights into the dynamics of m6A epitranscriptome: hybrid-seq identifies novel mRNAs of the m6A writers METTL3/14."],"pmcid":["PMC11457658"],"pubmed_authors":["Athanasopoulou K","Adamopoulos PG","Scorilas A"],"additional_accession":[]},"is_claimable":false,"name":"New insights into the dynamics of m6A epitranscriptome: hybrid-seq identifies novel mRNAs of the m6A writers METTL3/14.","description":"<b>Background:</b> N6-methyladenosine (m6A), a prevalent mRNA modification, is dynamically regulated by methyltransferases, including METTL3 and METTL14.<b>Materials & methods:</b> In the current study, we employed a custom hybrid-seq method to identify novel <i>METTL3</i>/<i>14</i> transcripts, explore their protein-coding capacities and predict the putative role of the METTL isoforms.<b>Results:</b> Demultiplexing of the hybrid-seq barcoded datasets unraveled the expression patterns of the newly identified mRNAs in major malignancies as well as in non-malignant cells, providing a deeper understanding of the methylation pathways. Open reading frame query revealed novel METTL3/14 isoforms, broadening our perspective for the structural diversity within METTL family.<b>Conclusion:</b> Our fi","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024","modification":"2026-04-08T15:18:00.288Z","creation":"2026-04-08T04:27:29.574Z"},"accession":"S-EPMC11457658","cross_references":{"pubmed":["39225157"],"doi":["10.1080/17501911.2024.2390818"]}}