{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xu H"],"funding":["NICHD NIH HHS","NCCIH NIH HHS","NIDA NIH HHS","NIAID NIH HHS","NHLBI NIH HHS","NINDS NIH HHS","NIGMS NIH HHS"],"pagination":["4946-4963.e17"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11458255"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["187(18)"],"pubmed_abstract":["The choroid plexus (ChP) is a vital brain barrier and source of cerebrospinal fluid (CSF). Here, we use longitudinal two-photon imaging in awake mice and single-cell transcriptomics to elucidate the mechanisms of ChP regulation of brain inflammation. We used intracerebroventricular injections of lipopolysaccharides (LPS) to model meningitis in mice and observed that neutrophils and monocytes accumulated in the ChP stroma and surged across the epithelial barrier into the CSF. Bi-directional recruitment of monocytes from the periphery and, unexpectedly, macrophages from the CSF to the ChP helped eliminate neutrophils and repair the barrier. Transcriptomic analyses detailed the molecular steps accompanying this process and revealed that ChP epithelial cells transiently specialize to nurture immune cells, coordinating their recruitment, survival, and differentiation as well as regulation of the tight junctions that control the permeability of the ChP brain barrier. Collectively, we provide a mechanistic understanding and a comprehensive roadmap of neuroinflammation at the ChP brain barrier."],"journal":["Cell"],"pubmed_title":["The choroid plexus synergizes with immune cells during neuroinflammation."],"pmcid":["PMC11458255"],"funding_grant_id":["T32 GM007753","R01 NS088566","T32 GM008313","RF1 DA048790","T32 NS007473","P50 HD105351","R01 NS129823","F32 NS136267","T32 GM144273","R01 HL162642","U54 HD090255","R01 AI168005","DP1 AT010971"],"pubmed_authors":["Lotfy P","Ordovas-Montanes J","Andermann ML","Chiu IM","Cui J","Webb M","Deng L","Xu H","Gelb S","Taylor M","Hehnly C","Lehtinen MK","Lidov HGW","Shipley FB","Zawadzki ME","Byer LIJ","Pragana A"],"additional_accession":[]},"is_claimable":false,"name":"The choroid plexus synergizes with immune cells during neuroinflammation.","description":"The choroid plexus (ChP) is a vital brain barrier and source of cerebrospinal fluid (CSF). Here, we use longitudinal two-photon imaging in awake mice and single-cell transcriptomics to elucidate the mechanisms of ChP regulation of brain inflammation. We used intracerebroventricular injections of lipopolysaccharides (LPS) to model meningitis in mice and observed that neutrophils and monocytes accumulated in the ChP stroma and surged across the epithelial barrier into the CSF. Bi-directional recruitment of monocytes from the periphery and, unexpectedly, macrophages from the CSF to the ChP helped eliminate neutrophils and repair the barrier. Transcriptomic analyses detailed the molecular steps accompanying this process and revealed that ChP epithelial cells transiently specialize to nurture immune cells, coordinating their recruitment, survival, and differentiation as well as regulation of the tight junctions that control the permeability of the ChP brain barrier. Collectively, we provide a mechanistic understanding and a comprehensive roadmap of neuroinflammation at the ChP brain barrier.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2026-06-30T03:28:16.822Z","creation":"2026-06-30T03:21:44.204Z"},"accession":"S-EPMC11458255","cross_references":{"pubmed":["39089253"],"doi":["10.1016/j.cell.2024.07.002"]}}