{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yajima H"],"funding":["MEXT | Japan Society for the Promotion of Science","MEXT | Japan Society for the Promotion of Science (JSPS)","Japan Agency for Medical Research and Development","MEXT | JST | Core Research for Evolutional Science and Technology","MEXT | JST | Core Research for Evolutional Science and Technology (CREST)","Japan Agency for Medical Research and Development (AMED)"],"pagination":["8574"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11458767"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Since 2019, SARS-CoV-2 has undergone mutations, resulting in pandemic and epidemic waves. The SARS-CoV-2 spike protein, crucial for cellular entry, binds to the ACE2 receptor exclusively when its receptor-binding domain (RBD) adopts the up-conformation. However, whether ACE2 also interacts with the RBD in the down-conformation to facilitate the conformational shift to RBD-up remains unclear. Herein, we present the structures of the BA.2.86 and the JN.1 spike proteins bound to ACE2. Notably, we successfully observed the ACE2-bound down-RBD, indicating an intermediate structure before the RBD-up conformation. The wider and mobile angle of RBDs in the up-state provides space for ACE2 to interact with the down-RBD, facilitating the transition to the RBD-up state. The K356T, but not N354-linked"],"journal":["Nature communications"],"pubmed_title":["Structural basis for receptor-binding domain mobility of the spike in SARS-CoV-2 BA.2.86 and JN.1."],"pmcid":["PMC11458767"],"funding_grant_id":["JP24jf0126002","JPMJCR20H8","JP20ae0101047","JPJSCCA20240006","JP223fa627009"],"pubmed_authors":["Nishiuchi T","Guo Z","Ogawa E","Plianchaisuk A","Asakura H","Irie T","Nasser H","Horinaka K","Kida I","Uriu K","Tsujino S","Deguchi S","Sadamas K","Kaku Y","Mohri H","Iida K","Yoshimura R","Iida M","Yoshimura K","Kita S","Ohari Y","Zahradnik J","Kosugi Y","Kuroda D","Saito A","Genotype to Phenotype Japan (G2P-Japan) Consortium","Sasaki J","Lytras S","Fukuhara T","Osujo R","Shishido K","Tsuda M","Motozono C","Sasaki-Tabata K","Takayama K","Nao N","Tanaka S","Matsuno K","Hashimoto R","Kosaka A","Maenaka K","Okumura K","Andrikopoulos P","Fujita S","Konar A","Yoshida I","Nishumura L","Anraku Y","Misawa N","Atarashi Y","Sato K","Ohsumi N","Fukuda T","Hemmi T","Wang L","Strange AP","Yasuda K","Ito J","Ito H","Kimura KT","Ferdous Z","Chiba M","Leong S","Toyoda M","Padilla-Blanco M","Kawano M","Li J","Mugita Y","Chen L","Sakamoto A","Ueno T","Ikeda T","Li W","Nakajima Y","Nakata Y","Futatsusako H","M Tolentino JE","Matsubara N","Hinay AA","Suzuki R","Yajima H","Suzuki S","Mimura Y","Suzuki T","Shimizu R","Yo MS","Sumita H","Mizuma K","Oda Y","Takahashi Y","Sawa H","Nakada-Nakura Y","Nagashima M","Takahashi O","Jonathan M","Tamura T","Watanabe Y","Kawakubo S","Usui K","Begum MSTM","Suganami M","Nakagawa S","Yasuhara N","Pan L","Kawabata R","Hashiguchi T","Saikruang W"],"additional_accession":[]},"is_claimable":false,"name":"Structural basis for receptor-binding domain mobility of the spike in SARS-CoV-2 BA.2.86 and JN.1.","description":"Since 2019, SARS-CoV-2 has undergone mutations, resulting in pandemic and epidemic waves. The SARS-CoV-2 spike protein, crucial for cellular entry, binds to the ACE2 receptor exclusively when its receptor-binding domain (RBD) adopts the up-conformation. However, whether ACE2 also interacts with the RBD in the down-conformation to facilitate the conformational shift to RBD-up remains unclear. Herein, we present the structures of the BA.2.86 and the JN.1 spike proteins bound to ACE2. Notably, we successfully observed the ACE2-bound down-RBD, indicating an intermediate structure before the RBD-up conformation. The wider and mobile angle of RBDs in the up-state provides space for ACE2 to interact with the down-RBD, facilitating the transition to the RBD-up state. The K356T, but not N354-linked","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-06-16T03:14:32.038Z","creation":"2025-04-04T01:54:39.173Z"},"accession":"S-EPMC11458767","cross_references":{"pubmed":["39375326"],"doi":["10.1038/s41467-024-52808-2"]}}