biostudies-literatureShan XNIDDK NIH HHSMichael Smith Foundation for Health ResearchNIDDK101378https://www.ebi.ac.uk/biostudies/studies/S-EPMC11459652biostudies-literatureUnknown18(5)<h4>Background & aims</h4>Addition of sialic acids (sialylation) to glycoconjugates is a common capping step of glycosylation. Our study aims to determine the roles of the overall sialylation in intestinal mucosal homeostasis.<h4>Methods</h4>Mice with constitutive deletion of intestinal epithelial sialylation (IEC Slc35a1^-/- mice) and mice with inducible deletion of sialylation in intestinal epithelium (TM-IEC Slc35a1^-/- mice) were generated, which were used to determine the roles of overall sialylation in intestinal mucosal homeostasis by ex vivo and mutiomics studies.<h4>Results</h4>IEC Slc35a1^-/- mice developed mild spontaneous microbiota-dependent colitis. Additionally, 30% of IEC Slc35a1^-/- mice had spontaneous tumors in the rectum greater tCellular and molecular gastroenterology and hepatologyAblation of Intestinal Epithelial Sialylation Predisposes to Acute and Chronic Intestinal Inflammation in Mice.PMC11459652R01 DK085691NitinGhosh SShan XLetef CLZandberg WKniffen DXia LBergstrom KSGao LRathore SShi HfalseAblation of Intestinal Epithelial Sialylation Predisposes to Acute and Chronic Intestinal Inflammation in Mice.<h4>Background & aims</h4>Addition of sialic acids (sialylation) to glycoconjugates is a common capping step of glycosylation. Our study aims to determine the roles of the overall sialylation in intestinal mucosal homeostasis.<h4>Methods</h4>Mice with constitutive deletion of intestinal epithelial sialylation (IEC Slc35a1^-/- mice) and mice with inducible deletion of sialylation in intestinal epithelium (TM-IEC Slc35a1^-/- mice) were generated, which were used to determine the roles of overall sialylation in intestinal mucosal homeostasis by ex vivo and mutiomics studies.<h4>Results</h4>IEC Slc35a1^-/- mice developed mild spontaneous microbiota-dependent colitis. Additionally, 30% of IEC Slc35a1^-/- mice had spontaneous tumors in the rectum greater t2024-01-01T00:00:00Z20242026-07-14T19:40:30.01Z2025-04-19T19:25:51.317ZS-EPMC114596523899246510.1016/j.jcmgh.2024.101378