biostudies-literatureLiu ZThe Scientific and Technological Innovation Project of China Academy of Chinese Medical SciencesFundamental Research Funds for the Central public welfare research institutes of ChinaNational Key Research and Development Program of ChinaBeijing Natural Science Foundation138https://www.ebi.ac.uk/biostudies/studies/S-EPMC11460136biostudies-literatureUnknown19(1)<h4>Background</h4>Fritillariae Cirrhosae Bulbus (FCB) is frequently adulterated with its closely related species due to personal or non-man made factors, leading to alterations in the composition of its constituents and compromising the efficacy of its products.<h4>Methods</h4>The specific single nucleotide polymorphisms (SNPs) were screened by comparing candidate barcodes of Fritillaria and verified by amplification and sequencing. Herb molecular quantification (Herb-Q) was established by detecting specific SNPs, and the methodological validation was performed. Quantitative standard curves were established for FCB mixed with each adulterated species, and the quantitative validity of this method was verified based on external standard substance. In addition, eight commercial Shedan ChuanbChinese medicineMolecular quantification of fritillariae cirrhosae bulbus and its adulterants.PMC114601362019YFC1710600CI2021A04106ZXKT23004ZZ15-YQ-033CI2024C003YN7244492Li XLiu ZYang ZJiang WFeng XPei YChen TfalseMolecular quantification of fritillariae cirrhosae bulbus and its adulterants.<h4>Background</h4>Fritillariae Cirrhosae Bulbus (FCB) is frequently adulterated with its closely related species due to personal or non-man made factors, leading to alterations in the composition of its constituents and compromising the efficacy of its products.<h4>Methods</h4>The specific single nucleotide polymorphisms (SNPs) were screened by comparing candidate barcodes of Fritillaria and verified by amplification and sequencing. Herb molecular quantification (Herb-Q) was established by detecting specific SNPs, and the methodological validation was performed. Quantitative standard curves were established for FCB mixed with each adulterated species, and the quantitative validity of this method was verified based on external standard substance. In addition, eight commercial Shedan Chuanb2024-01-01T00:00:00Z2024 Oct2026-06-18T09:48:31.106Z2025-04-19T19:27:56.712ZS-EPMC114601363938001410.1186/s13020-024-01010-z