biostudies-literatureGuo QNIA NIH HHSFoundation for the National Institutes of HealthNINDS NIH HHSNational Institutes of HealthNIH HHS67https://www.ebi.ac.uk/biostudies/studies/S-EPMC11460197biostudies-literatureUnknown19(1)<h4>Introduction</h4>Heparin binding proteins (HBPs) with roles in extracellular matrix assembly are strongly correlated to β-amyloid (Aβ) and tau pathology in Alzheimer's disease (AD) brain and cerebrospinal fluid (CSF). However, it remains challenging to detect these proteins in plasma using standard mass spectrometry-based proteomic approaches.<h4>Methods</h4>We employed heparin-affinity chromatography, followed by off-line fractionation and tandem mass tag mass spectrometry (TMT-MS), to enrich HBPs from plasma obtained from AD (n = 62) and control (n = 47) samples. These profiles were then correlated to Aβ, tau and phosphorylated tau (pTau) CSF biomarkers and plasma pTau181 from the same individuals, as well as a consensus brain proteome network to assess the overlap with AD brain pathMolecular neurodegenerationHeparin-enriched plasma proteome is significantly altered in Alzheimer's disease.PMC11460197U01 AG061357P30 AG066511P30AG066511AMP-AD 2.0RF1 AG062181U01AG061357RF1AG062181U01 NS128433Ping LXu KDuong DMRoberts BRLevey AIGuo QGolde TEFox EJSeyfried NTShantaraman ADammer EBLah JJYin LJohnson ECBfalseHeparin-enriched plasma proteome is significantly altered in Alzheimer's disease.<h4>Introduction</h4>Heparin binding proteins (HBPs) with roles in extracellular matrix assembly are strongly correlated to β-amyloid (Aβ) and tau pathology in Alzheimer's disease (AD) brain and cerebrospinal fluid (CSF). However, it remains challenging to detect these proteins in plasma using standard mass spectrometry-based proteomic approaches.<h4>Methods</h4>We employed heparin-affinity chromatography, followed by off-line fractionation and tandem mass tag mass spectrometry (TMT-MS), to enrich HBPs from plasma obtained from AD (n = 62) and control (n = 47) samples. These profiles were then correlated to Aβ, tau and phosphorylated tau (pTau) CSF biomarkers and plasma pTau181 from the same individuals, as well as a consensus brain proteome network to assess the overlap with AD brain path2024-01-01T00:00:00Z2024 Oct2026-07-15T17:43:53.567Z2026-07-07T03:10:47.293ZS-EPMC114601973938002110.1186/s13024-024-00757-1