biostudies-literatureLee HCNational Research Foundation of KoreaRP95285https://www.ebi.ac.uk/biostudies/studies/S-EPMC11460976biostudies-literatureUnknown13The gram-negative bacterium lipopolysaccharide (LPS) is frequently administered to generate models of systemic inflammation. However, there are several side effects and no effective treatment for LPS-induced systemic inflammation. PEGylated PDZ peptide based on zonula occludens-1 (ZO-1) was analyzed for its effects on systemic inflammation induced by LPS. PDZ peptide administration led to the restoration of tissue injuries (kidney, liver, and lung) and prevented alterations in biochemical plasma markers. The production of pro-inflammatory cytokines was significantly decreased in the plasma and lung BALF in the PDZ-administered mice. Flow cytometry analysis revealed the PDZ peptide significantly inhibited inflammation, mainly by decreasing the population of M1 macrophages, and neutrophils (eLifeLPS-induced systemic inflammation is suppressed by the PDZ motif peptide of ZO-1 <i>via</i> regulation of macrophage M1/M2 polarization.PMC114609762021R1A4A10313802023R1A2C3002483Lee HCPark YMPark SHJeong HMKim JShin GLim SIShim JSong KSfalseLPS-induced systemic inflammation is suppressed by the PDZ motif peptide of ZO-1 <i>via</i> regulation of macrophage M1/M2 polarization.The gram-negative bacterium lipopolysaccharide (LPS) is frequently administered to generate models of systemic inflammation. However, there are several side effects and no effective treatment for LPS-induced systemic inflammation. PEGylated PDZ peptide based on zonula occludens-1 (ZO-1) was analyzed for its effects on systemic inflammation induced by LPS. PDZ peptide administration led to the restoration of tissue injuries (kidney, liver, and lung) and prevented alterations in biochemical plasma markers. The production of pro-inflammatory cytokines was significantly decreased in the plasma and lung BALF in the PDZ-administered mice. Flow cytometry analysis revealed the PDZ peptide significantly inhibited inflammation, mainly by decreasing the population of M1 macrophages, and neutrophils (2024-01-01T00:00:00Z2024 Oct2026-07-14T20:39:20.88Z2025-04-04T02:05:06.41ZS-EPMC114609763937756810.7554/eLife.95285