biostudies-literatureZheng SSXiamen Municipal Bureau of Science and TechnologyNatural Science Foundation of Fujian Province1499-1515https://www.ebi.ac.uk/biostudies/studies/S-EPMC11461582biostudies-literatureUnknown18(5)<h4>Background</h4>Chromobox Homolog 1 (CBX1) plays a crucial role in the pathogenesis of numerous diseases, including the evolution and advancement of diverse cancers. The role of CBX1 in pan-cancer and its mechanism in hepatocellular carcinoma (HCC), however, remains to be further investigated.<h4>Methods</h4>Bioinformatics approaches were harnessed to scrutinize CBX1's expression profile, its association with tumor staging, and its potential impact on patient outcomes across various cancers. Single-cell RNA sequencing data facilitated the investigation of CBX1 expression patterns at the individual cell level. The CBX1 expression levels in HCC and adjacent non-tumor tissues were quantified through Real-Time Polymerase Chain Reaction (RT-PCR), Western Blotting (WB), and ImmunohistochemicaHepatology internationalCBX1 is involved in hepatocellular carcinoma progression and resistance to sorafenib and lenvatinib via IGF-1R/AKT/SNAIL signaling pathway.PMC114615823502Z20224ZD10812022J05332Wu JFHuang CZhang DZheng SSWu WXHu JWZhang BHfalseCBX1 is involved in hepatocellular carcinoma progression and resistance to sorafenib and lenvatinib via IGF-1R/AKT/SNAIL signaling pathway.<h4>Background</h4>Chromobox Homolog 1 (CBX1) plays a crucial role in the pathogenesis of numerous diseases, including the evolution and advancement of diverse cancers. The role of CBX1 in pan-cancer and its mechanism in hepatocellular carcinoma (HCC), however, remains to be further investigated.<h4>Methods</h4>Bioinformatics approaches were harnessed to scrutinize CBX1's expression profile, its association with tumor staging, and its potential impact on patient outcomes across various cancers. Single-cell RNA sequencing data facilitated the investigation of CBX1 expression patterns at the individual cell level. The CBX1 expression levels in HCC and adjacent non-tumor tissues were quantified through Real-Time Polymerase Chain Reaction (RT-PCR), Western Blotting (WB), and Immunohistochemica2024-01-01T00:00:00Z2024 Oct2025-04-26T20:12:52.358Z2025-04-06T16:23:27.767ZS-EPMC114615823876928610.1007/s12072-024-10696-0